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Expression of trehalose synthase gene from Pseudomonas putida P06 in Pichia pastoris

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dc.contributor.author Tengfei, Wang
dc.contributor.author Dai, Kun
dc.contributor.author Jia, Shiru
dc.date.accessioned 2023-01-20T06:00:47Z
dc.date.available 2023-01-20T06:00:47Z
dc.date.issued 2014-05-07
dc.identifier.citation Tengfei, W., Liu, H., Dai, K., Wang, R., & Jia, S. (2014). Expression of trehalose synthase gene from Pseudomonas putida P06 in Pichia pastoris. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16237
dc.description.abstract The trehalose synthase (TreS) gene from Pseudomonas putida P06 was successfully ligated with pPICZaA expression vector by the EcoRI and XbaI and was transformed into Pichia pastoris GS115 by electrotransformation. The trehalose synthase gene was fused to the genome of Pichia pastoris GS115 and was controlled by AOX1 promoter. The TreS protein was successfully expressed in intracellularly. SDS-PAGE results illustrated that a specificity protein band was observed at about 76 kDa. The cell lysates could convert 60% maltose into trehalose at 50°C and pH 7.5 in 10% maltose substrate for 24 h. The Pichia pastorisas exogenous gene expression host is safer to produce endotoxin free TreS than E.coli. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Trehalose synthase en_US
dc.subject Pseudomonas putida P06 en_US
dc.subject Pichia pastoris en_US
dc.subject cloning and expression en_US
dc.title Expression of trehalose synthase gene from Pseudomonas putida P06 in Pichia pastoris en_US
dc.type Article en_US


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