dc.contributor.author |
Luo, Yan’e |
|
dc.contributor.author |
Mu, Tingzhen |
|
dc.contributor.author |
Fan, Daidi |
|
dc.date.accessioned |
2023-01-20T06:01:09Z |
|
dc.date.available |
2023-01-20T06:01:09Z |
|
dc.date.issued |
2014-05-08 |
|
dc.identifier.citation |
Mu, T., & Fan, D. (2014). Preparation of a low-cost minimal medium for engineered Escherichia coli with high yield of human-like collagen II. Pakistan Journal of Pharmaceutical Sciences, 27. |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16238 |
|
dc.description.abstract |
In order to reduce the production cost of human-like collagen (HLC), a minimal medium was introduced. On
the base of Design of experiments (DOE), especially Plackett-Burman design and central composite design, a modified minimal medium that could give a high yield of HLC was developed. The optimum minimal medium for engineered E. coli BL21 ∆ptsG contained 6.11g/L of glucose, 5.82g/L of (NH4) 2SO4, 1.80×10-4g/L of thiamine and 3.00×10-2L of trace element solution, the other ingredients were same to that in M9 medium. And the HLC production of ptsG mutant reached to 0.26g/L in this optimized minimal medium, which approached to 0.27g/L produced by the strain without deleting ptsG gene in an optimized complex medium. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi |
en_US |
dc.subject |
E. coli |
en_US |
dc.subject |
a modified minimal medium |
en_US |
dc.subject |
human-like collagen II |
en_US |
dc.subject |
ptsG deletion |
en_US |
dc.title |
Preparation of a low-cost minimal medium for engineered Escherichia coli with high yield of human-like collagen II |
en_US |
dc.type |
Article |
en_US |