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Preparation of a low-cost minimal medium for engineered Escherichia coli with high yield of human-like collagen II

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dc.contributor.author Luo, Yan’e
dc.contributor.author Mu, Tingzhen
dc.contributor.author Fan, Daidi
dc.date.accessioned 2023-01-20T06:01:09Z
dc.date.available 2023-01-20T06:01:09Z
dc.date.issued 2014-05-08
dc.identifier.citation Mu, T., & Fan, D. (2014). Preparation of a low-cost minimal medium for engineered Escherichia coli with high yield of human-like collagen II. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16238
dc.description.abstract In order to reduce the production cost of human-like collagen (HLC), a minimal medium was introduced. On the base of Design of experiments (DOE), especially Plackett-Burman design and central composite design, a modified minimal medium that could give a high yield of HLC was developed. The optimum minimal medium for engineered E. coli BL21 ∆ptsG contained 6.11g/L of glucose, 5.82g/L of (NH4) 2SO4, 1.80×10-4g/L of thiamine and 3.00×10-2L of trace element solution, the other ingredients were same to that in M9 medium. And the HLC production of ptsG mutant reached to 0.26g/L in this optimized minimal medium, which approached to 0.27g/L produced by the strain without deleting ptsG gene in an optimized complex medium. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject E. coli en_US
dc.subject a modified minimal medium en_US
dc.subject human-like collagen II en_US
dc.subject ptsG deletion en_US
dc.title Preparation of a low-cost minimal medium for engineered Escherichia coli with high yield of human-like collagen II en_US
dc.type Article en_US


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