PASTIC Dspace Repository

The Study on the factors affecting transformation efficiency of E. coli competent cells

Show simple item record

dc.contributor.author Liu, Xiaofeng
dc.contributor.author Liu, Lin
dc.contributor.author Wang, Yonggang
dc.contributor.author Wang, Xiaoli
dc.contributor.author Ma, Yanling
dc.contributor.author Li, Yunchun
dc.date.accessioned 2023-01-20T06:01:58Z
dc.date.available 2023-01-20T06:01:58Z
dc.date.issued 2014-05-10
dc.identifier.citation Liu, X., Liu, L., Wang, Y., Wang, X., Ma, Y., & Li, Y. (2014). The Study on the factors affecting transformation efficiency of E. coli competent cells. Cell, 27(3 Suppl), 679-84. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16240
dc.description.abstract The preparation of competent cell is the central step of bacteria transformation and has a great impact on transformation efficiency of nucleic acid. The aim of the research was to study the factors such as ionic species, strain types, plasmid concentration, ice-bath time and incubation time. The result shows that ionic species is fatal to the transformation efficiency, preparation of competent cells by monovalent ions (Li+ 、Na+ 、K+ ) and tervalent ion (Al3+) do not have capacity of transformation, preparation of competent cells by bivalent ion (Ba2+, Ca2+, Mn2+, Mg2+, Sr2+) has capacity of transformation. On the whole, the efficiency of Ca2+ was found to be the optimum bivalent ion, following by Sr2+>Mn2+>Mg2+>Ba2+. On the other factors that affecting transformation efficiency, the transformation efficiency was the best when plasmid concentration was 100ng/mL, and ice-bath time should be controlled at about 30 min and incubate time selected as 60 min. Experiment of natural transformation later proves the existence of the phenomenon of natural transformation and it’s also related to factors such as calcium chloride concentration, plasmid concentration and others. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject competent cell en_US
dc.subject different ion en_US
dc.subject transformation efficiency en_US
dc.subject Escherichia coli en_US
dc.title The Study on the factors affecting transformation efficiency of E. coli competent cells en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account