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The effect of enzyme digestion time on the detection of diatom species

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dc.contributor.author Yu, Zhengliang
dc.contributor.author Liu, Chao
dc.contributor.author Wang, Huijun
dc.contributor.author Chen, Ling
dc.contributor.author Hu, Sunlin
dc.contributor.author Zhao, Jian
dc.contributor.author Wang, Huipin
dc.contributor.author Zhou, Yu
dc.contributor.author Xie, Weibing
dc.date.accessioned 2023-01-20T06:02:57Z
dc.date.available 2023-01-20T06:02:57Z
dc.date.issued 2014-05-12
dc.identifier.citation Yu, Z., Liu, C., Wang, H., Chen, L., Hu, S., Zhao, J., ... & Xie, W. (2014). The effect of enzyme digestion time on the detection of diatom species. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16242
dc.description.abstract This study is aimed at detecting diatom in lung, liver and kidney tissues using PCR - DHPLC technology after different periods of enzyme digestion to assess the effect of enzyme digestion on the detection of diatom species. Twenty Randomly selected experimental rabbits were drowned at the same place. Their liver, kidney, and lung tissues were removed for sampling. After the extraction of DNA from the samples, amplification was conducted with specific primers of the SSU gene of diatom. Then, an analysis was performed with agarose gel electrophoresis and DHPLC. Within 2 h-8 h, the amount of the diatom species found in the lung gradually increased over time and was statistically significant (P<0.05). After 8 h, with enzyme digestion, the amount of the diatom species found in lung showed no significant increase (P>0.05). However, as for the liver and kidney, within 2h-6h, the amount of the diatom species gradually increased over time and was statistically significant (P<0.05). After 6h, the fig. did not present significant growth (P>0.05). The amount of the diatom species found in the organs after different periods of digestion time had significant differences, which provides a reference for the detection of diatoms and also, has a good application prospect in the forensic identification of drowning. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Forensic medicine en_US
dc.subject drowning en_US
dc.subject enzyme digestion en_US
dc.subject detection of diatom en_US
dc.subject SSU gene en_US
dc.subject PCR-DHPLC en_US
dc.title The effect of enzyme digestion time on the detection of diatom species en_US
dc.type Article en_US


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