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Identification and characterization of a helicase-like protein encoded by a Thermus siphoviridae phage 4 gene

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dc.contributor.author Zhang, Qi
dc.contributor.author Li, Qiupeng
dc.contributor.author Ji, Xiuling
dc.contributor.author Hong, Wei
dc.contributor.author Dong, Zhiyang
dc.contributor.author Wei, Yunlin
dc.contributor.author Lin, Lianbing
dc.date.accessioned 2023-01-20T06:03:37Z
dc.date.available 2023-01-20T06:03:37Z
dc.date.issued 2014-05-14
dc.identifier.citation Zhang, Q., Li, Q., Ji, X., Hong, W., Dong, Z., Wei, Y., & Lin, L. (2014). Identification and characterization of a helicase-like protein encoded by a Thermus siphoviridae phage 4 gene. Pakistan Journal of Pharmaceutical Sciences, 27(3), 703-711. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16244
dc.description.abstract DNA helicases are essential motor proteins that unwind duplex DNA to yield the transient single-stranded DNA intermediates required for replication, recombination, and repair. As laboratory model strains of thermostable bacteria, the roles of Thermus have been studied and discussed extensively. In this study, one gene (ORF42) encoding a helicase-like protein of TSP4 (Thermus Siphoviridae phage 4) was identified and characterized. The results showed that ORF42 protein shared a higher homology to the DnaB helicases of Thermus bacteriophages P74-26 and P24-46. DNA helicase assay and atomic force microscopy (AFM) revealed that ORF42 protein was an Mg2+-dependent helicase with ATPase activity and involved in DNA unwinding. These evidences indicated that ORF42 protein, homologue of DnaB, probably acts as a helicase in TSP4. This study will not only contribute to explore the co-evolution of Thermus phages and their hosts but also shed a new light on the “arm-race” pattern between Thermus and its predator (TSP4), providing a basis for the theoretical investigations of new generation bacteriophage therapy. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Thermostable bacteria en_US
dc.subject Bacteriophage en_US
dc.subject Thermus siphoviridae phage 4 en_US
dc.subject DnaB Helicase en_US
dc.title Identification and characterization of a helicase-like protein encoded by a Thermus siphoviridae phage 4 gene en_US
dc.type Article en_US


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