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Cytotoxicity of hollow silica nanoparticles loaded with photosensitizes on huh-7 cells

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dc.contributor.author Pei, Dongni
dc.contributor.author Xiong, Li
dc.contributor.author Lin, Liangwu
dc.contributor.author Zhong, Dewu
dc.date.accessioned 2023-01-20T06:04:24Z
dc.date.available 2023-01-20T06:04:24Z
dc.date.issued 2014-05-18
dc.identifier.citation Pei, D., Xiong, L., Lin, L., & Zhong, D. (2014). Cytotoxicity of hollow silica nanoparticles loaded with photosensitizes on huh-7 cells. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16246
dc.description.abstract To observe the cytotoxic effect of the photodynamic therapy mediated by the traditional photosensitizer polyhematoporphyrin (C34H38N4NaO5, Photosan-II Photosan-II was loaded into HSNP by one-step wet chemical, PS) and hollow silica nanoparticles (HSNP) loaded PS on Huh-7 cells and compare the cytotoxic effects. -based synthetic route. The cellular viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Apoptotic and necrotic cells were measured by flow cytometry. The suitable drug concentrations of free PS and HSNP loaded PS on Huh-7 cells were 20mg/L and 5mg/L respectively, and the suitable incubation time were 4 h and 2 h respectively. Under the same drug concentration, the survival rates of free PS and HSNP loaded PS were 62.46%±1.93% and 6.54%±1.24% (P<0.05). Under the same drug concentration and incubation time, the total rates of apoptosis necrosis caused by free PS and HSNP loaded PS mediated PDT were respectively 22.00%±2.24% and 87.23%±2.1% (P<0.05). PS-loaded HSNP mediated PDT can inhibit proliferation of cancer cells and induce apoptosis more quickly and effectively. As the loading system of PS, HSNP can make the photosensitizer have stronger solubility and drug concentration efficiency, which can significantly improve the therapeutic effect of PDT. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Photodynamic therapy en_US
dc.subject Photosan-II en_US
dc.subject hollow silica nanoparticles en_US
dc.subject Huh-7 en_US
dc.subject hepatocellular carcinoma en_US
dc.title Cytotoxicity of hollow silica nanoparticles loaded with photosensitizes on huh-7 cells en_US
dc.type Article en_US


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