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St. John’s Wort increases brain seotonin synthesis by inhibiting hepatic tryptophan 2, 3 dioxygenase activity and its gene expression in stressed rats

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dc.contributor.author Samina Bano
dc.contributor.author Iffat Ara
dc.contributor.author Kausar Saboohi
dc.contributor.author Tariq Moattar
dc.contributor.author Chaoudhry, Bushra
dc.date.accessioned 2023-01-20T06:10:00Z
dc.date.available 2023-01-20T06:10:00Z
dc.date.issued 2014-09-05
dc.identifier.citation St. John’s Wort increases brain seotonin synthesis by inhibiting hepatic tryptophan 2, 3 dioxygenase activity and its gene expression in stressed rats en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16258
dc.description.abstract We aimed to investigate the effects of herbal St. John’s Wort (SJW) on transcriptional regulation of hepatic tryptophan 2, 3 - dioxygenase (TDO) enzyme activity and brain regional serotonin (5-HT) levels in rats exposed to forced swim test (FST). TDO mRNA expression was quantified using real-time reverse transcription polymerase chain (RT-PCR) reaction and brain regional indoleamines were determined by high performance liquid chromatography coupled to fluorescence detector. Behavioral analysis shows significant reduction in immobility time in SJW (500mg/kg/ml) administered rats. It was found that pretreatment of SJW to rats did not prevent stress-induced elevation in plasma corticosterone levels however it increases serotonin synthesis by virtue of inhibiting hepatic TDO enzyme activity and its gene expression, ascertaining the notion that there exists an inverse relationship between hepatic TDO enzyme activity and brain 5-HT. The drug also decreases serotonin turnover in all the brain areas (hypothalamus, hippocampus amygdala) in stressed rats endorsing its monoamine oxidase inhibition property. Inhibition of TDO enzyme activity and its gene expression by the drug provides new insights for the development of therapeutic interventions for stress elated mental illnesses. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy, University of Karachi en_US
dc.subject Tryptophan 2 en_US
dc.subject 3 - dioxygenase en_US
dc.subject serotonin en_US
dc.subject tryptophan metabolism en_US
dc.subject TDO mRNA expression en_US
dc.subject St. John’s Wort en_US
dc.title St. John’s Wort increases brain seotonin synthesis by inhibiting hepatic tryptophan 2, 3 dioxygenase activity and its gene expression in stressed rats en_US
dc.type Article en_US


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