PASTIC Dspace Repository

Cloning, expression and characterization of the maltooligosyl trehalose synthase from the archaeon Sulfolobus tokodaii

Show simple item record

dc.contributor.author Zhao, Meng
dc.contributor.author Xu, Xiangqun
dc.contributor.author Yang, Shaolong
dc.contributor.author Liu, Tianming
dc.contributor.author Liu, Bo
dc.date.accessioned 2023-01-20T06:51:09Z
dc.date.available 2023-01-20T06:51:09Z
dc.date.issued 2018-03-01
dc.identifier.citation Zhao, M., Xu, X., Yang, S., Liu, T., & Liu, B. (2018). Cloning, expression and characterization of the maltooligosyl trehalose synthase from the archaeon Sulfolobus tokodaii. Pakistan Journal of Pharmaceutical Sciences, 31. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16294
dc.description.abstract The maltooligosyl trehalose synthase gene from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7 was cloned and the recombinant peotein was expressed in E. coli. The protein was purified to homogeneity by nickel column chromatography. The archaeal enzyme could catalyze an intramolecular transglycosylation reaction and convert the glycosidic bond at the reducing end of dextrins from α-1, 4 (reducing end) into α-1, 1 (non-reducing end). The most suitable temperature was 75°C and the optimal pH was 5. Substrate specificity investigation revealed that maltodextrin and maltooligosaccharide were used as substrates by the enzyme but maltose, chitooligosaccharide, sucrose and βcyclodextrin weren’t used. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Maltooligosyl trehalose synthase en_US
dc.subject Sulfolobus tokodaii en_US
dc.subject trehalose en_US
dc.subject archaeon en_US
dc.title Cloning, expression and characterization of the maltooligosyl trehalose synthase from the archaeon Sulfolobus tokodaii en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account