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Evaluation of antibiotic resistant bacteria in underground drinking water and transfer of their resistant character to normal flora of the body

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dc.contributor.author Mehboob Alam
dc.contributor.author Khan, Naqab
dc.contributor.author Khurram Rehman
dc.contributor.author Khan, Samiullah
dc.contributor.author Zahid Rasul Niazi
dc.contributor.author Kifayatullah Shah
dc.contributor.author Natasha Baloch
dc.contributor.author Khan, Barkat Ali
dc.date.accessioned 2023-01-20T06:52:50Z
dc.date.available 2023-01-20T06:52:50Z
dc.date.issued 2018-03-09
dc.identifier.citation Alam, M., Khan, N., Rehman, K., Khan, S., Niazi, Z. R., Shah, K., ... & Khan, B. A. (2018). Evaluation of antibiotic resistant bacteria in underground drinking water and transfer of their resistant character to normal flora of the body. Pakistan Journal of Pharmaceutical Sciences, 31. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16300
dc.description.abstract The untreated surface water for drinking and domestic use is an alarming situation to public health especially in prevalence of antibiotics resistant bacteria. This investigation aimed to isolate and identify the antibiotic resistance bacteria in underground water samples in district Dera Ismail Khan, Pakistan. The underground water samples were collected from four different places using hand pumps (Khyber town, riverside, Gomal University and united town). Cultured on nutrient agar media, identified by Gam staining and biochemical tests. There after antibiotic resistance assay were performed by measuring zone of inhibition of different antibiotics by disc diffusion method. Six different bacterial colonies were isolated and identified as Enterobacteriaceae, Serriata specie, Proteues, Pseudomonas, all these bacterial colonies were 33% resistant to chloramphenicol with and 100% resistant to amoxicillin. Some colonies were also considered as resistant, according to the criteria of National Committee for Clinical Records (NCCL) that less than 10mm zone of inhibition are considered as resistant. Subsequently, the chloramphenicol resistance bacteria were analyzed for their ability to transfer resistant gene to sensitive bacteria. In in-vitro method, an isolate M1b (resistant) was found capable to transfer resistance gene to M1a isolate (sensitive) in nutrient rich environment. It was concluded that antibiotics resistance bacteria found in underground water, moreover capable of transferring the antibiotic resistant character to suitable recipient i.e. normal flora of the body or to other pathogens by conjugation. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Staining en_US
dc.subject chloramphenicol en_US
dc.subject antibiotic resistance en_US
dc.subject conjugation en_US
dc.subject resistant pathogens en_US
dc.title Evaluation of antibiotic resistant bacteria in underground drinking water and transfer of their resistant character to normal flora of the body en_US
dc.type Article en_US


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