dc.contributor.author |
Bin Asad, Muhammad Hassham Hassan |
|
dc.contributor.author |
Arooj Fatima Asad |
|
dc.contributor.author |
Sumbal Bibi |
|
dc.contributor.author |
Kaleem Ullah |
|
dc.contributor.author |
Tariq Javed |
|
dc.contributor.author |
Majeed Ullah |
|
dc.contributor.author |
Atif Ali |
|
dc.contributor.author |
Zia-ur-Rahman Qureshi |
|
dc.contributor.author |
Muhammad Imran Amirzada |
|
dc.contributor.author |
Yasser MSA Al-Kahraman |
|
dc.contributor.author |
Syed Muhammad Farid Hasan |
|
dc.contributor.author |
Jean-Marc Sabatier |
|
dc.contributor.author |
Albert Rizvanov |
|
dc.date.accessioned |
2023-01-20T06:54:10Z |
|
dc.date.available |
2023-01-20T06:54:10Z |
|
dc.date.issued |
2018-03-13 |
|
dc.identifier.citation |
Asad, M. H. H. B., Asad, A. F., Bibi, S., Ullah, K., Javed, T., Ullah, M., ... & Rizvanov, A. (2018). Lethal toxic Dose (ip LD 50), total protein contents and comparative hemolytic potential of (99m Tc labeled & non-labeled) Naja naja karachiensis venom. Pakistan Journal of Pharmaceutical Sciences, 31. |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16302 |
|
dc.description.abstract |
Recent recognition about snake bite envenomation on June, 2017 as neglected tropical disease under categoryA by World Health Organization advocated again its undeniable importance. Present circumstances reasoned to work on a neglected subspecies of Naja naja, i.e., Naja naja karachiensis (N. n. karachensis) has been documented for frequent deaths in Pakistan. In this study median lethal toxic dose (LD50) was determined intraperitoneally in Swiss albino mice and was found to be 2.0µg/g (2.0mg/kg) equal in potency to Naja pallida (red spitting African cobra). Total protein contents (188±0.011µg / 200µg of dry weight) were high enough (94%) to represent an arsenal of proteins. Furthermore, 99mTc was labeled 99.9% with venom and didn’t find to alter hemolytic activity of venom in dose dependent manner at 125µg/ml (p℘0.5), 250 µg/ml (p℘0.1) and 500 µg/ml (p℘0.1) when compared with its crude form. Present work will pave the way for proteomics study in effective production of antidote against specific species of snakes as dare demand of it has been felt since long period of time in Pakistan. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi |
en_US |
dc.subject |
Intraperitoneal LD50 |
en_US |
dc.subject |
Naja naja karachiensis |
en_US |
dc.subject |
protein contents |
en_US |
dc.subject |
hemolytic activity |
en_US |
dc.title |
Lethal toxic Dose (i.p LD50), total protein contents and comparative hemolytic potential of (99mTc labeled & non-labeled) Naja naja karachiensis venom |
en_US |
dc.type |
Article |
en_US |