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Lethal toxic Dose (i.p LD50), total protein contents and comparative hemolytic potential of (99mTc labeled & non-labeled) Naja naja karachiensis venom

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dc.contributor.author Bin Asad, Muhammad Hassham Hassan
dc.contributor.author Arooj Fatima Asad
dc.contributor.author Sumbal Bibi
dc.contributor.author Kaleem Ullah
dc.contributor.author Tariq Javed
dc.contributor.author Majeed Ullah
dc.contributor.author Atif Ali
dc.contributor.author Zia-ur-Rahman Qureshi
dc.contributor.author Muhammad Imran Amirzada
dc.contributor.author Yasser MSA Al-Kahraman
dc.contributor.author Syed Muhammad Farid Hasan
dc.contributor.author Jean-Marc Sabatier
dc.contributor.author Albert Rizvanov
dc.date.accessioned 2023-01-20T06:54:10Z
dc.date.available 2023-01-20T06:54:10Z
dc.date.issued 2018-03-13
dc.identifier.citation Asad, M. H. H. B., Asad, A. F., Bibi, S., Ullah, K., Javed, T., Ullah, M., ... & Rizvanov, A. (2018). Lethal toxic Dose (ip LD 50), total protein contents and comparative hemolytic potential of (99m Tc labeled & non-labeled) Naja naja karachiensis venom. Pakistan Journal of Pharmaceutical Sciences, 31. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16302
dc.description.abstract Recent recognition about snake bite envenomation on June, 2017 as neglected tropical disease under categoryA by World Health Organization advocated again its undeniable importance. Present circumstances reasoned to work on a neglected subspecies of Naja naja, i.e., Naja naja karachiensis (N. n. karachensis) has been documented for frequent deaths in Pakistan. In this study median lethal toxic dose (LD50) was determined intraperitoneally in Swiss albino mice and was found to be 2.0µg/g (2.0mg/kg) equal in potency to Naja pallida (red spitting African cobra). Total protein contents (188±0.011µg / 200µg of dry weight) were high enough (94%) to represent an arsenal of proteins. Furthermore, 99mTc was labeled 99.9% with venom and didn’t find to alter hemolytic activity of venom in dose dependent manner at 125µg/ml (p℘0.5), 250 µg/ml (p℘0.1) and 500 µg/ml (p℘0.1) when compared with its crude form. Present work will pave the way for proteomics study in effective production of antidote against specific species of snakes as dare demand of it has been felt since long period of time in Pakistan. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Intraperitoneal LD50 en_US
dc.subject Naja naja karachiensis en_US
dc.subject protein contents en_US
dc.subject hemolytic activity en_US
dc.title Lethal toxic Dose (i.p LD50), total protein contents and comparative hemolytic potential of (99mTc labeled & non-labeled) Naja naja karachiensis venom en_US
dc.type Article en_US


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