PASTIC Dspace Repository

Structural and functional annotation of citrate synthase from Aspergillus niger ANJ-120

Show simple item record

dc.contributor.author Ghulam Mustafa
dc.contributor.author Rawaba Arif
dc.contributor.author Shazia Anwer Bukhari
dc.contributor.author Muhammad Ali
dc.contributor.author Sumaira Sharif
dc.contributor.author Asia Atta
dc.date.accessioned 2023-01-20T06:56:14Z
dc.date.available 2023-01-20T06:56:14Z
dc.date.issued 2018-03-16
dc.identifier.citation Mustafa, G., Arif, R., Bukhari, S. A., Ali, M., Sharif, S., & Atta, A. (2018). Structural and functional annotation of citrate synthase from Aspergillus niger ANJ-120. Pakistan journal of pharmaceutical sciences, 31. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16309
dc.description.abstract Citrate synthase (CS) is involved in citric acid biosynthesis which is a well-established metabolic pathway. The condensation of acetyl-CoA with oxaloacetate is catalyzed by CS. Citric acid (CA) has a number of applications in pharmaceutical industry. CA in combination with bicarbonates is used as an effervescent in the preparations of tablets and powders. It has also been used as an anticoagulant and acidulant to form mild astringent. In current study, detailed structural and functional analyses of CS protein were carried out using various bioinformatics tools. Structural modeling was also done by building 3D model of CS from Aspergillus niger ANJ-120 using Modeller 9.16 software. The 3D Model was then evaluated using different online approaches. Furthermore, superimposition of query and template structures, Root Mean Squared Deviation and visualization of generated model were done through UCSF Chimera 1.5.3. Even though various roles of CS protein were already known and verified experimentally, here we presented a structural analysis of CS protein. The structural investigation of CS protein will be helpful for protein engineering strategies and understanding the interactions among proteins. Due to large number of applications, the production of citric acid by A. niger and its bioinformatics studies will offer substantial improvement in commercial scale intensification of this useful product. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Aspergillus niger en_US
dc.subject citrate synthase en_US
dc.subject annotation en_US
dc.subject citric acid en_US
dc.subject homology modeling en_US
dc.title Structural and functional annotation of citrate synthase from Aspergillus niger ANJ-120 en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account