PASTIC Dspace Repository

Development and validation of HPLC analytical method for Nepafenac in ophthalmic dosage form (suspension)

Show simple item record

dc.contributor.author Shahnaz Usman
dc.contributor.author Muhammad Akram
dc.contributor.author Asif Aziz
dc.contributor.author Asif Aziz
dc.contributor.author Omar Abdulraheem Sarheed
dc.date.accessioned 2023-01-20T07:01:07Z
dc.date.available 2023-01-20T07:01:07Z
dc.date.issued 2014-09-20
dc.identifier.citation Usman, S., Akram, M., Aziz, A., Ramesh, V., & Sarheed, O. A. (2014). Development and validation of HPLC analytical method for Nepafenac in ophthalmic dosage form (suspension). Pakistan journal of pharmaceutical sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16320
dc.description.abstract The aim of the present study was to develop and validate an analytical method for the estimation of nepafenac as a raw material as well as in dosage form (suspension) by using reverse phase high performance liquid chromatographic (RP-HPLC). The target was to obtain an easy, rapid, reproducible as well as a rugged ethod. The HPLC system that was used in the proposed study was LC-20AD liquid chromatograph equipped with SPD-20A UVVIS detector. The separation was performed on C18 column which was attached with loop 20µl. lution was done at ambient temperature with a mobile phase consisting of acetonitrile: Water (40: 60v/v) at a flow rate of 1ml/min and at a wavelength of 254 nm. The proposed method was validated as per the ICH guidelines. The retention time for nepafenac was 7.49 minutes (% CV=0.0076). The percentage coefficient variation (CV) of six consecutive peak areas of injections was 0.34% with tailing factor 1.76. The peak area responses were linear within the concentration range of 0.078- 20.0µg/ml (R2 =0.9993). The sensitivity of the method could be evaluated by limits of detection (LOD) (0.0195µg/ml) and limits of quantitation (LOQ) .039µg/ml). Nepafenac drug is s in its diluent that could see by intra-day (% CV =0.45-1.96) and inter-day variation (%CV=0.173-1.898%). The accuracy and recovery results of 80%, 100% and 120% were 97.40% to 102.10% with % CV of 0.3201% to 1.3496%. The robustness and ruggedness of the method are significantly broader and is reproducible. It could be used as a more convenient, efficient, easy and time saving method for the analysis of drug in raw material as well as in dosage form (ophthalmic suspension). en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy, University of Karachi en_US
dc.subject Anti-inflammatory drug, NSAIDs en_US
dc.subject High Performance Liquid Chromatography en_US
dc.subject ophthalmic suspension en_US
dc.subject Quantitative analysis en_US
dc.subject Validation en_US
dc.title Development and validation of HPLC analytical method for Nepafenac in ophthalmic dosage form (suspension) en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account