dc.contributor.author |
Faisal Sultan |
|
dc.contributor.author |
Muhammad Harris Shoaib |
|
dc.contributor.author |
Rabia Ismail Yousuf |
|
dc.contributor.author |
Farrukh Rafiq Ahmed |
|
dc.contributor.author |
Faseeh A Salam |
|
dc.contributor.author |
Muhammad Iqbal Nasiri |
|
dc.contributor.author |
Muhammad Atif Khan |
|
dc.contributor.author |
Saeed Manzoor |
|
dc.date.accessioned |
2023-01-20T07:01:46Z |
|
dc.date.available |
2023-01-20T07:01:46Z |
|
dc.date.issued |
2014-09-22 |
|
dc.identifier.citation |
Sultan, F., Shoaib, M. H., Yousuf, R. I., Ahmed, F. R., Salam, F. A., Nasiri, M. I., ... & Manzoor, S. (2014). Simultaneous quantitation of aspirin, amlodipine and simvastatin in a fixed dose combination of encapsulated tablet formulation by HPLC-UV method. Pakistan Journal of Pharmaceutical Sciences, 27. |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16322 |
|
dc.description.abstract |
A high-pressure liquid chromatography (HPLC-UV) based simple and specific method for simultaneous quantitative determination of aspirin, amlodipine besylate and simvastatin in a capsule formulation has been developed and validated according to ICH guidelines. Chromatographic separation of the three drugs was carried out by aSpherisorbODS2 reverse phase column (4.6 x 250 mm; 5 µm) using amobile phase, which consisted of 70: 30 (v/v) mixture of acetonitrile and triethylamine phosphate buffer (pH 3; 0.015 M) with final pH adjusted to 2.5 using dilute ortho-phosphoric acid, at a flow rate of 1mL/min. The eluents were detected at UV wavelength of 237 nm and the retention times for aspirin, amlodipine besylate and simvastatin were ~2.7 mins, ~6.1 mins and ~10.5mins, respectively. This method is suitable and specific for the three drugs and was found to be linear (R2 >0.995), accurate, specific, reproducible and robust in the concentration range of 375 to 1125mcg/ml for aspirin, 25 to 75mcg/ml for amlodipine besylate and 50 to 150mcg/ml for simvastatin. This simple and convenient method could be easily utilized for the characterization and quantitation of the three rugs in a single formulation for combination therapy of cardiovascular diseases. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy, University of Karachi |
en_US |
dc.subject |
Aspirin |
en_US |
dc.subject |
amlodipine besylate |
en_US |
dc.subject |
simvastatin |
en_US |
dc.subject |
HPLC-UV |
en_US |
dc.subject |
analysis |
en_US |
dc.subject |
validation |
en_US |
dc.title |
Simultaneous quantitation of aspirin, amlodipine and simvastatin in a fixed dose combination of encapsulated tablet formulation by HPLCUV method |
en_US |
dc.type |
Article |
en_US |