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Simultaneous quantitation of aspirin, amlodipine and simvastatin in a fixed dose combination of encapsulated tablet formulation by HPLCUV method

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dc.contributor.author Faisal Sultan
dc.contributor.author Muhammad Harris Shoaib
dc.contributor.author Rabia Ismail Yousuf
dc.contributor.author Farrukh Rafiq Ahmed
dc.contributor.author Faseeh A Salam
dc.contributor.author Muhammad Iqbal Nasiri
dc.contributor.author Muhammad Atif Khan
dc.contributor.author Saeed Manzoor
dc.date.accessioned 2023-01-20T07:01:46Z
dc.date.available 2023-01-20T07:01:46Z
dc.date.issued 2014-09-22
dc.identifier.citation Sultan, F., Shoaib, M. H., Yousuf, R. I., Ahmed, F. R., Salam, F. A., Nasiri, M. I., ... & Manzoor, S. (2014). Simultaneous quantitation of aspirin, amlodipine and simvastatin in a fixed dose combination of encapsulated tablet formulation by HPLC-UV method. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16322
dc.description.abstract A high-pressure liquid chromatography (HPLC-UV) based simple and specific method for simultaneous quantitative determination of aspirin, amlodipine besylate and simvastatin in a capsule formulation has been developed and validated according to ICH guidelines. Chromatographic separation of the three drugs was carried out by aSpherisorbODS2 reverse phase column (4.6 x 250 mm; 5 µm) using amobile phase, which consisted of 70: 30 (v/v) mixture of acetonitrile and triethylamine phosphate buffer (pH 3; 0.015 M) with final pH adjusted to 2.5 using dilute ortho-phosphoric acid, at a flow rate of 1mL/min. The eluents were detected at UV wavelength of 237 nm and the retention times for aspirin, amlodipine besylate and simvastatin were ~2.7 mins, ~6.1 mins and ~10.5mins, respectively. This method is suitable and specific for the three drugs and was found to be linear (R2 >0.995), accurate, specific, reproducible and robust in the concentration range of 375 to 1125mcg/ml for aspirin, 25 to 75mcg/ml for amlodipine besylate and 50 to 150mcg/ml for simvastatin. This simple and convenient method could be easily utilized for the characterization and quantitation of the three rugs in a single formulation for combination therapy of cardiovascular diseases. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy, University of Karachi en_US
dc.subject Aspirin en_US
dc.subject amlodipine besylate en_US
dc.subject simvastatin en_US
dc.subject HPLC-UV en_US
dc.subject analysis en_US
dc.subject validation en_US
dc.title Simultaneous quantitation of aspirin, amlodipine and simvastatin in a fixed dose combination of encapsulated tablet formulation by HPLCUV method en_US
dc.type Article en_US


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