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REPORT Screening for toxigenic Escherichia coli in stool samples of diarrhoeal patients by polymerase chain reaction

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dc.contributor.author Benish Nawaz
dc.contributor.author Anum AlI
dc.contributor.author Muhammad Noman Syed
dc.contributor.author Khan, Abdul Basit
dc.date.accessioned 2023-01-20T07:02:34Z
dc.date.available 2023-01-20T07:02:34Z
dc.date.issued 2014-09-25
dc.identifier.citation Nawaz, B., Ali, A., Syed, M. N., & Khan, A. B. (2014). Screening for toxigenic Escherichia coli in stool samples of diarrhoeal patients by polymerase chain reaction. Pakistan Journal of Pharmaceutical Sciences, 27. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16325
dc.description.abstract Escherichia coli (E. coli) are normal flora of the intestines of most animals, including humans. Most strains are harmless and beneficial to host by preventing the establishment of pathogenic bacteria within the intestine. However, some E. coli strains can cause a wide variety of intestinal and extra-intestinal diseases, such as diarrhoea, urinary tract infections, septicaemia, neonatal meningitis and renal complications. Several virulence factors including toxins, adhesins, serine proteases, etc. have been reported in these highly adapted clones. The present study was designed to enumerate toxin genotype through PCR assay in local clinical isolates of E. coli. A total of 37 E. coli strains were collected from different clinical laboratories of Karachi and examined for the presence of shiga toxin 1 (stx1) and shiga toxin 2 (stx2) genes of Eenterohemorrhagic E. Coli (EHEC) and heat stable (st) and healt labile (lt) toxin genes of enterotoxigenic E. Coli (ETEC). It was observed that 16 strains out of 37 carried one or more type of toxin genes. The presence of stx1 gene was significantly higher as it was positive in 10 isolates compared to others toxins. Two in above stx1 positive strains were also carrying for stx2 gene. Six out of 37 isolates were positive for lt gene, and none of the strains are carrying st gene. Although, the study was carried out with fewer isolates, yet it demonstrated the trend of dispersion of toxin genes and findings can be used to correlate the gastro-intestinal infections and their complications in Pakistan. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy, University of Karachi en_US
dc.subject EHEC en_US
dc.subject EHEC en_US
dc.subject Diarrhoea en_US
dc.subject Shiga toxins en_US
dc.subject Heat-labile toxin, en_US
dc.subject Heat-stable toxin en_US
dc.title REPORT Screening for toxigenic Escherichia coli in stool samples of diarrhoeal patients by polymerase chain reaction en_US
dc.type Article en_US


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