dc.contributor.author |
Yan, Huiyu |
|
dc.contributor.author |
Tao, Lina |
|
dc.contributor.author |
Qu, Xiaoyu |
|
dc.contributor.author |
Zhou, Liting |
|
dc.contributor.author |
Zhang, Sixi |
|
dc.date.accessioned |
2023-01-20T07:09:01Z |
|
dc.date.available |
2023-01-20T07:09:01Z |
|
dc.date.issued |
2018-05-19 |
|
dc.identifier.citation |
Yan, H., Tao, L., Qu, X., Zhou, L., & Zhang, S. (2018). Quantitative determination of mogroside V in rat plasma by LC-MS/MS and its application to a pharmacokinetic study. Pakistan Journal of Pharmaceutical Sciences, 31(3). |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16346 |
|
dc.description.abstract |
Mogroside V is the most abundant (approximately 0.50%) cucurbitane-type triterpene glycoside in Siraitia
grosvenorii and exhibits significant antitussive, expectorant, anti-carcinogenic, and anti-inflammatory effects. A sensitive, robust and selective liquid chromatography tandem with mass spectrometry (LC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of mogroside V in rat plasma. Samples were prepared through an one-step deproteinization procedure with 250 µL of methanol to a 75-µL plasma sample. Plasma samples were effectively separated on a Shiseido Capcell Pak UG120 C18 column (2.0 × 50mm, 3.0µm) using a mobile phase consisting of methanol: water (60:40, v/v) with an isocratic elution program. The running time for each sample was 7.0 min and the elution times of mogroside V and IS were 2.0 and 4.8 min, respectively. The detection relied on a triplequadrupole tandem with mass spectrometer equipped with negative-ion electrospray ionization interface by selectedreaction monitoring (SRM) of the transitions at m/z 1285.6 → 1123.7 for mogroside V and m/z 1089.6 → 649.6 for IS. The calibration curve was linear over the range of 96.0–96000ng/mL with a limit of quantitation (LOQ) of 96.0ng/mL. Intra-day and inter-day precisions were both <10.1%. Mean recovery and matrix effect of mogroside V in plasma were in
the range of 91.3-95.7% and 98.2-105.0%, respectively. This method was successfully applied in the pharmacokinetic study of mogroside V after intravenous or intraperitoneal administration of 1.12mg/kg mogroside V in rats. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi |
en_US |
dc.subject |
Mogroside V |
en_US |
dc.subject |
LC-MS/MS |
en_US |
dc.subject |
Pharmacokinetic study |
en_US |
dc.title |
Quantitative determination of mogroside V in rat plasma by LCMS/MS and its application to a pharmacokinetic study |
en_US |
dc.type |
Article |
en_US |