Abstract:
ABSTRACT
Cytotoxicity, the possible selective activity upon HL60 as well as the anti-proliferation effect of local health
supplement wheatgrass and mixture of fibers were investigated in vitro using various cancerous cell line and
normal blood cell culture. The IC50 of wheatgrass-treated HL60 (17.5 ± 1.1, 12.5 ± 0.3, and 16 ± 0.5 microgram/ml for 24, 48 and 72 h, respectively) and fibers-treated HL60 (86.0 ± 5.5, 35.0 ± 2.5, and 52.5 ± 4.5
microgram/ml for 24, 48 and 72 h, respectively) showed that both extracts possessed optimum effect after 48
hours of treatment. No significant cytotoxic effect was observed on other type of cells. For trypan blue dye
exclusion method, wheatgrass reduced the number of viable cells by 13.5% (±1.5), 47.1% (±3.6), and 64.9%
(±2.7) after 24, 48 and 72 h exposure, respectively. Mixture of fibers reduced the number of viable cells by
36.4% (±2.3), 57.1% (±3.1), and 89.0% (±3.4) after 24, 48 and 72 h exposure, respectively, indicated that
necrosis is also an alternative to the apoptotic mechanism of cell death. Annexin-V/propidium iodide staining
revealed that both extracts induced apoptosis where early apoptosis had been detected concurrently with the
reduction of percentage of cell viability. Cell cycle analysis revealed that in HL60, the percentage of apoptosis
increased with time (wheatgrass: 16.0% ± 2.4, 45.3% ± 3.4 and 39.6% ± 4.1; mixture of fibers: 14.6% ± 1.8,
45.4% ± 2.3 and 45.9% ± 1.2) after exposure for 24, 48 and 72 h, respectively at the concentration of 100
microgram/ml and showed optimum effect at 48 hours. Thus, these health products can be a potential alternative supplement for leukaemia patients.
