dc.contributor.author |
Seo, Chang-Seob |
|
dc.contributor.author |
Kim, Jung Hoon |
|
dc.contributor.author |
Shin, Hyeun-Kyoo |
|
dc.date.accessioned |
2023-01-20T07:23:03Z |
|
dc.date.available |
2023-01-20T07:23:03Z |
|
dc.date.issued |
2014-07-11 |
|
dc.identifier.citation |
Seo, C. S., Kim, J. H., & Shin, H. K. (2014). Simultaneous determination of liquiritin, nodakenin and glycyrrhizinin Guibi-tang, a traditional herbal prescription by HPLC-PDA. Pakistan Journal of Pharmaceutical Sciences, 27(4). |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16373 |
|
dc.description.abstract |
A simple, convenient, rapid and accurate high-performance liquid chromatographic (HPLC) method was
established for the simultaneous determination about three ingredients of a traditional herbal prescription, Guibi-tang (GBT): liquiritin (1), nodakenin (2) and glycyrrhizin (3). Chromatographic analysis on the three components was separated within 35 min on a Gemini C18 column and maintained at 40°C. The mobile phase consisted of water with 1.0% (v/v) acetic acid (solvent A) and acetonitrile with 1.0% (v/v) acetic acid (solvent B) in gradient mode at a flow-rate of 1.0 mL/min. Chromatograms were acquired at 254, 280 and 330 nm in a photodiode array (PDA) detector. The calibration curves showed excellent linearity (R2 =1.0000). The average recovery of three compounds was ≥93.5%, with a relative standard deviation (RSD) of ≤2.0%. The intra-day and inter-day precision (RSD) of the three components were 0.04%-3.04% and 0.11%–2.48%, respectively. The contents of compounds 1–3 in GBT were 1.09-1.10, 2.35-2.37 and 0.81-0.82 mg/g, respectively. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy, University of Karachi |
en_US |
dc.subject |
Simultaneous determination |
en_US |
dc.subject |
Guibi-tang; herbal prescription |
en_US |
dc.subject |
HPLC–PDA |
en_US |
dc.subject |
validation |
en_US |
dc.title |
Simultaneous determination of liquiritin, nodakenin and glycyrrhizinin Guibi-tang, a traditional herbal prescription by HPLC-PDA |
en_US |
dc.type |
Article |
en_US |