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Development and validation of simultaneous estimation method for curcumin and piperine by RP-UFLC

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dc.contributor.author Shanmugam Ramaswamy
dc.contributor.author Gowthamarajan Kuppuswamy
dc.contributor.author Priyanka Dwarampudi L
dc.contributor.author Madhuri Kadiyala
dc.contributor.author Elango Kannan
dc.date.accessioned 2023-01-20T07:27:29Z
dc.date.available 2023-01-20T07:27:29Z
dc.date.issued 2014-07-23
dc.identifier.citation Ramaswamy, S., Kuppuswamy, G., Dwarampudi, P., Kadiyala, M., Menta, L., & Kannan, E. (2014). Development and validation of simultaneous estimation method for curcumin and piperine by RP-UFLC. Pak J Pharm Sci, 27(4), 901-6. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16388
dc.description.abstract Curcumin and piperine are proven for their potent medicinal benefits to treat various diseases and they are most commonly used combination in various Indian systems of medicine such as Ayurveda, Siddha and Unani. The objective of the present work is to develop a simultaneous estimation of curcumin and piperine by reverse phase Ultrafast liquid chromatographic (RP-UFLC) method. The chromatographic separation was performed on a C8 column (250 x 4.6 mm, 5µ i.d.) stationary phase using a mobile phase of 25mM potassium dihydrogen ortho phosphate buffer (pH 3.5) and acetonitrile (30: 70 v/v) at a flow rate of lml/min at detection wave length of 280nm. The calibration curve was plotted in the concentration range of 0-2200ng/ml and found to be linear for both curcumin (r2 =0.996) and piperine (r2 =0.999). The method was validated for parameters such as accuracy, sensitivity, precision, linearity, specificity, ruggedness and robustness as per ICH guidelines. The developed simple, precise and specific method can be used as a quality control tool for qualitative and quantitative estimation of curcumin and piperine in various food products, herbal medicines and nutraceuticals. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy, University of Karachi en_US
dc.subject Curcumin en_US
dc.subject piperine en_US
dc.subject ICH guidelines en_US
dc.subject RP-UFLC en_US
dc.subject herbal medicinal products en_US
dc.title Development and validation of simultaneous estimation method for curcumin and piperine by RP-UFLC en_US
dc.type Article en_US


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