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EFFECTS OF APOMORPHINE ON LOCOMOTIVE ACTIVITY AND MONOAMINE METABOLISM: A DOSE RELATED STUDY

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dc.contributor.author HUMA IKRAM
dc.contributor.author SHOAIB AHMAD
dc.contributor.author HALEEM, DARAKHSHAN JABEEN
dc.date.accessioned 2023-01-20T07:27:46Z
dc.date.available 2023-01-20T07:27:46Z
dc.date.issued 2011-07-20
dc.identifier.citation Ikram, H., Ahmad, S., & Haleem, D. J. (2011). Effects of apomorphine on locomotive activity and monoamine metabolism: a dose related study. Pakistan Journal of Pharmaceutical Sciences, 24(3). en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16389
dc.description.abstract We have monitored dose dependent effects of apomorphine on motor activity and monoamine metabolism. Behavioral sensitization and craving, which develop upon repeated treatment with dopamine receptor agonist apomorphine, are major limitations of the therapeutic use of apomorphine in Parkinson’s patients. Effects of single (intraperitoneal) injection of apomorphine at different doses (i.e., 1.0, 2.0 & 4.0 mg/kg) on exploration in a novel environment (open field) and locomotion in a familiar environment (home cage) were investigated. Results show significantly enhanced activity in home cage (monitored 5min post injection) in a dose dependent manner. However, no significant influence of apomorphine on exploration of open field was observed in the present study (monitored 15min and 40min post injection). Animals were decapitated 1 hr post apomorphine injection and whole brains of animals were collected and stored at -70ο C. Biogenic amines (i.e., 5- Hydroxytryptamine and dopamine) and metabolites (i.e., Dihydroxyphenylacetic acid, Homovanillic acid & 5- Hydroxyindoleacetic acid) were estimated by reverse phase High Performance Liquid Chromatography with electrochemical detector (HPLC-EC). Effect of low (1.0mg/kg) dose of apomorphine was found to be nonsignificant on 5-Hydroxytryptamine (5-HT), 5-Hydroxyindoleacetic acid (5-HIAA) and dopamine (DA) levels. Moderate (2.0 mg/kg) dose of drug increased (p<0.05) levels of Homovanillic acid (HVA). Whereas, high (4.0 mg/kg) dose of apomorphine decreased Dihydroxyphenylacetic acid (DOPAC) levels. Results could be helpful in elucidating the effect of apomorphine at different doses and its implication for extending therapeutics in Parkinson’s and related disorders. en_US
dc.language.iso en en_US
dc.publisher Karachi:Pakistan Journal of Pharmaceutical Sciences, university of Karachi. en_US
dc.subject Apomorphine en_US
dc.subject CNS stimulant en_US
dc.subject serotonin en_US
dc.subject dopamine en_US
dc.subject behavioral sensitization en_US
dc.title EFFECTS OF APOMORPHINE ON LOCOMOTIVE ACTIVITY AND MONOAMINE METABOLISM: A DOSE RELATED STUDY en_US
dc.type Article en_US


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