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Efficacy of Azadirachta indica organic extracts against clinical methicillin resistant Staphylococcus aureus isolates

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dc.contributor.author Jaweria Anwar
dc.contributor.author Muhammad Asif Zahoor
dc.contributor.author Muhammad Kashif Zahoor
dc.contributor.author Abu Baker Siddique
dc.contributor.author Zeeshan Nawaz
dc.contributor.author Muhammad Hidayat Rasool
dc.contributor.author Muhammad Usman Qamar
dc.contributor.author Muhammad Waseem
dc.contributor.author Syed Zajif Hussain
dc.contributor.author Aysha Yasmin
dc.date.accessioned 2023-01-20T07:31:11Z
dc.date.available 2023-01-20T07:31:11Z
dc.date.issued 2018-07-06
dc.identifier.citation Anwar, J., Zahoor, M. A., Zahoor, M. K., Siddique, A. B., Nawaz, Z., Rasool, M. H., ... & Yasmin, A. (2018). Efficacy of Azadirachta indica organic extracts against clinical methicillin resistant Staphylococcus aureus isolates. Pakistan journal of pharmaceutical sciences, 31(4), 1485-1488. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16400
dc.description.abstract In current study we investigated the efficacy of organic extracts of Azadirachta indica leaves against Methicillin Resistant Staphylococcus aureus (MRSA) clinical isolates. For this purpose fresh leaves were used to prepare ethanol, methanol and chloroform extract. Secondly, a cross sectional study was conducted to isolate MRSA in clinical samples from patients having surgical/ non-surgical wounds from Allied Hospital and District Head Quarter Hospital, Faisalabad. The S. aureus isolates were initially identified by biochemical characterization, followed by identification of MRSA using cefoxitin disc diffusion test that was finally confirmed by genomic amplification of mecA gene, responsible for resistance. All MRSA isolates were tested to find vancomycin resistant S. aureus (VRSA) using E-strips (M.I.C. EvaluatorTM, Oxide, UK). The data showed an overall 37% prevalence of S. aureus including 56.75% clinical MRSA isolates while none of the isolated S. aureus showed resistance to vancomycin. The antimicrobial activity was measured as mean zone of inhibition for each extract against all MRSA isolates and it was found as 15.38±2.26, 16.09±3.09 and 17.42±2.48 for methanol, ethanol and chloroform extracts respectively. Chloroform extract showed significantly high antimicrobial activity against MRSA isolates. Altogether, the current study exposed the high prevalence of MRSA isolates from tertiary care hospitals. However, all MRSA isolates were found susceptible to organic extracts of A. indica leaves. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject MRSA en_US
dc.subject VRSA en_US
dc.subject antimicrobial en_US
dc.subject wound infection en_US
dc.title Efficacy of Azadirachta indica organic extracts against clinical methicillin resistant Staphylococcus aureus isolates en_US
dc.type Article en_US


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