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Enhanced production of streptokinase from Streptococcus agalactiae EBL-31 by response surface methodology

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dc.contributor.author Arooj Arshad
dc.contributor.author Muhammad Anjum Zia
dc.contributor.author Muhammad Asgher
dc.contributor.author Joyia, Faiz Ahmad
dc.contributor.author Muhammad Arif
dc.date.accessioned 2023-01-20T07:36:39Z
dc.date.available 2023-01-20T07:36:39Z
dc.date.issued 2018-07-23
dc.identifier.citation Arshad, A., Zia, M. A., Asgher, M., Joyia, F. A., & Arif, M. (2018). Enhanced production of streptokinase from Streptococcus agalactiae EBL-31 by response surface methodology. Pakistan Journal of Pharmaceutical Sciences, 31. en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16418
dc.description.abstract Streptokinase (SK) is a fibrinolytic protein used for the treatment of cardiovascular disorders. In the present study, enhanced production of SK was achieved by determining the optimum fermentation conditions for the maximum growth of Streptococcus agalactiae EBL-31 using response surface methodology (RSM). Four process variables (pH, temperature, incubation time and inoculum size) with five levels were evaluated in 30 experimental runs. Central composite rotatable design (CCRD) was employed to predict the effect of independent variables on SK activity. The statistical evaluation by ANOVA showed that the model was fit as the effect of single factors, quadratic effects and most of the interactions among variables. The value ofR2 (0.9988) indicated the satisfactory interaction between the experimental and predicted responses. Furthermore, the model F value (902.67) and coefficient of variation (1.92) clearly showed that the model is significant (p =>0.0001). The functional activity of SK was determined by spectrophotometric analysis and maximum SK production was obtained at pH-7.0, temperature- 37.5o C, an incubation time of 36 hours and 2.5 mL inoculum size. Hence it was concluded that the optimization of culture conditions through RSM increases the production of SK by 2.01-fold. Production of SK by fermentation is an economical choice to be used for the treatment of cardiovascular diseases. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Cardiovascular disorders en_US
dc.subject Fermentation en_US
dc.subject Response surface methodology en_US
dc.subject Streptokinase en_US
dc.title Enhanced production of streptokinase from Streptococcus agalactiae EBL-31 by response surface methodology en_US
dc.type Article en_US


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