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Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans

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dc.contributor.author Li, Zhenjing
dc.contributor.author Zhang, Yanan
dc.contributor.author Lin, Lin
dc.contributor.author Cui, Haiyan
dc.contributor.author Wang, Tiantian
dc.contributor.author Chen, Mianhua
dc.contributor.author Wang, Changlu
dc.date.accessioned 2023-01-20T09:15:59Z
dc.date.available 2023-01-20T09:15:59Z
dc.date.issued 2018-11-24
dc.identifier.citation Li, Z., Zhang, Y., Lin, L., Cui, H., Wang, T., Chen, M., & Wang, C. (2018). Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of en_US
dc.identifier.issn 1011-601X
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/16491
dc.description.abstract We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans. A Box–Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments. For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power. Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w). One fraction (FRP-2) was isolated and purified, and its characteristics were analyzed. The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose. The sugar units are present both in the α-configuration and β-configuration. Moreover, FRP-2 exhibited certain antiultraviolet activity to C. elegans when the polysaccharide concentration ranged between 0.05mg/mL and 0.20mg/mL. en_US
dc.language.iso en en_US
dc.publisher Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi en_US
dc.subject Anti-ultraviolet activity en_US
dc.subject C. elegans en_US
dc.subject cellulase-assisted extraction en_US
dc.subject Flammulina velutipes root en_US
dc.subject polysaccharides en_US
dc.title Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans en_US
dc.type Article en_US


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