dc.contributor.author |
Li, Zhenjing |
|
dc.contributor.author |
Zhang, Yanan |
|
dc.contributor.author |
Lin, Lin |
|
dc.contributor.author |
Cui, Haiyan |
|
dc.contributor.author |
Wang, Tiantian |
|
dc.contributor.author |
Chen, Mianhua |
|
dc.contributor.author |
Wang, Changlu |
|
dc.date.accessioned |
2023-01-20T09:15:59Z |
|
dc.date.available |
2023-01-20T09:15:59Z |
|
dc.date.issued |
2018-11-24 |
|
dc.identifier.citation |
Li, Z., Zhang, Y., Lin, L., Cui, H., Wang, T., Chen, M., & Wang, C. (2018). Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of |
en_US |
dc.identifier.issn |
1011-601X |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/16491 |
|
dc.description.abstract |
We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides
from the root of Flammulina velutipes on Caenorhabditis elegans. A Box–Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments. For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67°C enzymolysis temperature, 80s microwave time, and 144 W microwave power. Under optimal conditions, the actual measured value of the yield was 2.01% (w/w) and the predicted value was 2.06% (w/w). One fraction (FRP-2) was isolated and purified, and its characteristics were analyzed. The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose. The sugar units are present both in the α-configuration and β-configuration. Moreover, FRP-2 exhibited certain antiultraviolet activity to C. elegans when the polysaccharide concentration ranged between 0.05mg/mL and 0.20mg/mL. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Karachi: Faculty of Pharmacy & Pharmaceutical Sciences University of Karachi |
en_US |
dc.subject |
Anti-ultraviolet activity |
en_US |
dc.subject |
C. elegans |
en_US |
dc.subject |
cellulase-assisted extraction |
en_US |
dc.subject |
Flammulina velutipes root |
en_US |
dc.subject |
polysaccharides |
en_US |
dc.title |
Cellulase-assisted extraction and anti-ultraviolet activity of polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans |
en_US |
dc.type |
Article |
en_US |