Abstract:
A spectrophotometric method has been developed for the quantitative determination of natural and ring-substituted indoles by complexing with maleic anhydride. The coloured complex exhibits absorbance maxima in the region 490-530 nm. The RSD of the method is 2.6%. The method is specific and simple and thus ideal for the routine analysis of indole compounds by absorbance measurements in the visible region. The analysis of each indole complex can be carried out within 15 minutes.