Abstract:
Conditions for in vitro cultures of calli from root, cotyledon and hypocotyl explants of chickpea (Cicer arietinum L. cv. CM 72) in M.S. medium were established. Total phenolics, phenylalanine ammonia-lyase (PAL) and polyphenol oxidases (PPO) were estimated quantitatively from main callus to 6th sub-culture. The activity of PAL and PPO showed a linear increase upto third sub-culture and then a remarkable decrease from fourth to sixth sub-culture was observed. rIhe data of phenolics was not in conformity with PAL and PPO activity. A gradual increase in total phenolics from main to sach subculture was observed. The data obtained from PAL, PP() and total phenolics correlates with the callus growth.
