Abstract:
Methods were developea 101 cauus tnauction and efficient plant regeneration from mature embryos and immature inflorescences of basmati rice (Oyza sativa L. cv. Basmati-370). Callusing from seeds of rice was observed in MS or N6 media with various levels of 2,4-D. Somatic embryogenesis was achieved in both media however, response was better in the presence of Kinetin (2 mg l- ). Using field grown immature inflorescences and embryos induction of callus and regeneration was observed. Cell suspension cultures were initiated from 2-3 months old calli ofB-370. A fast growing and finely dispersed cell line of Basmati-370 was established. Regeneration of plants was achieved in MS-O (no hormone).
