ESTABLISHMENT OF REGENERATING CALLI AND CELL SUSPENSION LINE OF BASMATI RICE (ORYZ4 SATIVA L. CV. B.370)

Abstract

Methods were developea 101 cauus tnauction and efficient plant regeneration from mature embryos and immature inflorescences of basmati rice (Oyza sativa L. cv. Basmati-370). Callusing from seeds of rice was observed in MS or N6 media with various levels of 2,4-D. Somatic embryogenesis was achieved in both media however, response was better in the presence of Kinetin (2 mg l- ). Using field grown immature inflorescences and embryos induction of callus and regeneration was observed. Cell suspension cultures were initiated from 2-3 months old calli ofB-370. A fast growing and finely dispersed cell line of Basmati-370 was established. Regeneration of plants was achieved in MS-O (no hormone).