Protoplasts from leaves, roots excised from young seedlings and calli induced from roots of seeds have been isolated with a digestion mixture containing 2.5% Macerozyme, 2.5% cellulase Onozuka-R40 and i% hemicellulase in 0.7% mannitol at pH 5.6 after 6 h incubation. Young leaves yielded highest amount of protoplasts and appeared to be a good source of plant material.
