ISOLATION AND YIELD OF PROTOPLASTS FROM DIFFERENT  TISSUES OF TRITICUM AESTIVUM IN STERILE CULTURE

dc.contributor.author	KHALIDA KHATOON
dc.date.accessioned	2023-02-02T06:21:22Z
dc.date.available	2023-02-02T06:21:22Z
dc.date.issued	1985-12-04
dc.identifier.citation	Khatoon, K. (1985). Isolation and yield of protoplasts from different tissues of Triticum aestivum in sterile culture. Pakistan Journal of Botany.	en_US
dc.identifier.issn	0556-2231
dc.identifier.uri	http://142.54.178.187:9060/xmlui/handle/123456789/16857
dc.description.abstract	Protoplasts from leaves, roots excised from young seedlings and calli induced from roots of seeds have been isolated with a digestion mixture containing 2.5% Macerozyme, 2.5% cellulase Onozuka-R40 and i% hemicellulase in 0.7% mannitol at pH 5.6 after 6 h incubation. Young leaves yielded highest amount of protoplasts and appeared to be a good source of plant material.	en_US
dc.language.iso	en	en_US
dc.publisher	Karachi: Pakistan Botanical Society	en_US
dc.title	ISOLATION AND YIELD OF PROTOPLASTS FROM DIFFERENT TISSUES OF TRITICUM AESTIVUM IN STERILE CULTURE	en_US
dc.type	Article	en_US