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A MICROPROPAGATION SYSTEM FOR CLONING OF HEMP (CANNABIS SATIVA L.) BY SHOOT TIP CULTURE

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dc.contributor.author Ren Wang
dc.contributor.author Li-Si He
dc.contributor.author Bing Xia
dc.contributor.author Tong, Jin-Feng
dc.contributor.author Ning Li
dc.contributor.author Feng Peng
dc.date.accessioned 2023-03-06T06:56:38Z
dc.date.available 2023-03-06T06:56:38Z
dc.date.issued 2009-04-20
dc.identifier.citation Wang, R., He, L. S., Xia, B., Tong, J. F., Li, N., & Peng, F. (2009). A micropropagation system for cloning of hemp (Cannabis sativa L.) by shoot tip culture. Pak. J. Bot, 41(2), 603-608. en_US
dc.identifier.issn 0556-3321
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/17470
dc.description.abstract This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·L-1sucrose, 5.5 g·L-1agar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·L-1TDZ, 0.1 mg·L-1NAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds were successfully rooted on MS medium supplemented with 0.1 mg·L-1IBA and 0.05 mg·L-1NAA resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes 35-42 days for acclimatized plantlet production. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society, University of Karachi en_US
dc.title A MICROPROPAGATION SYSTEM FOR CLONING OF HEMP (CANNABIS SATIVA L.) BY SHOOT TIP CULTURE en_US
dc.type Article en_US


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