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SCREENING OF ASPERGILLUS NIGER AND A. FLAVUS STRAINS FOR EXTRA CELLULAR ALPHA-AMYLASE ACTIVITY

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dc.contributor.author Sobiya Shafique
dc.contributor.author Rukshana Bajwa
dc.contributor.author Shaiza Shafique
dc.date.accessioned 2023-03-06T07:04:17Z
dc.date.available 2023-03-06T07:04:17Z
dc.date.issued 2009-04-20
dc.identifier.citation Shafique, S., Bajwa, R., & Shafique, S. (2009). Screening of Aspergillus niger and A. flavus strains for extra cellular alpha-amylase activity. Pak. J. Bot, 41(2), 897-905. en_US
dc.identifier.issn 0556-3321
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/17527
dc.description.abstract Five fungal strains of each of the two filamentous fungi viz., Aspergillus niger Van Tieghem and A. flavus Link ex Gray, indigenous to Pakistan, were screened for their alpha-amylase activity. Different selected strains were grown on two growth media viz., Potato Dextrose Agar (PDA) and Enzyme Production Medium (EPM), at three pH levels viz., 4.5, 5.5 and 6.5, in all the possible combinations. Performance of various strains was recorded in terms of hydrolyzing zone formation. Generally test strains exhibited their best performance on EPM at pH 4.5. On the basis of their best performance on solid media, strain 74 and strain 198 of A. niger and strain 209 and strain 231 of A. flavus were selected for periodic evaluation of their alpha-amylase activity in liquid medium, using shake flask technique. All the test strains exhibited their maximum alpha-amylase activity after 48 h incubation. en_US
dc.language.iso en en_US
dc.publisher Karachi: Pakistan Botanical Society, University of Karachi en_US
dc.title SCREENING OF ASPERGILLUS NIGER AND A. FLAVUS STRAINS FOR EXTRA CELLULAR ALPHA-AMYLASE ACTIVITY en_US
dc.type Article en_US


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