Abstract:
The optimum conditions for agar gel precipitation test were determined by using homologous (Rhabditis axei antigen vs Rhabditis axei amibody), heterlogous (Rhabditis axei antigen vs Toxocara l'illilorum antibody) and vice versa systems. Yernol buffer was replaced by normal saline (pH at 8.5) in the preparation of gel. Precipitation lines were observed against Toxocara Vitulorum antigen with antise_a prepared in rabbits against Rhabdi1is a.xei antigen. Best precipitation lines were seen after 5 days of incubation at 37°C. Similar observations were also recorded by using homologous system. Rhabditis axei shares some common antigen(s) with Toxocara vitulorum.
