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ELECTROPHORETIC PATTERN OF POLYPEPTIDES OF INFECTIOUS BURSAL DISEASE VACCINE VIRUS

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dc.contributor.author Aftab Ahmad Anjum
dc.contributor.author Iftikhar Hussain
dc.contributor.author Muhammad Ashfaque
dc.contributor.author Rizwana Tabassu
dc.contributor.author Talha Khalid Ahmad
dc.date.accessioned 2023-03-07T06:48:53Z
dc.date.available 2023-03-07T06:48:53Z
dc.date.issued 1997-10-06
dc.identifier.citation Hussain, I., Ashfaque, M., & Tabassure, R. (1997). Electrophoretic pattern of polypeptides of infectious bursal disease vaccine virus. PAKISTAN VETERINARY JOURNAL, 17, 183-186. en_US
dc.identifier.issn 0253-8313
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/17934
dc.description.abstract Four live vaccines and five field isolates of infectious bursal disease virus (IBDV) were analyzed for polypeptide profile using sodium dodecyle sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Field viruses were purified and concentrated through sucrose gradient centrifugation. All the five field IBDV contained five polypeptides with molecular weights (MW) 27.70, 32.70, 41.60, 63.09 and 91.20 KDa. Out of four vaccinal strains of IBDV, only one vaccinal strain contained five polypeptides with almost similar MW i.e.,26.0, 32.0, 40.8, 62.0 and 90.9 KDa and one extra band (MW 20.3 KDa). One vaccine virus had three polypeptides with MW 40.8, 62.0 and 90.9 KDa and the remaining two vaccines contained two polypeptides with MW 40.8 and 90.9 KDa. Results reveal that vaccinal strains of IBDV differ from local field isolates in the sense of low protein profile or missing of some polypeptides. en_US
dc.language.iso en en_US
dc.publisher Faisalabad: Faculty of Veterinary Science University of Agriculture Faisalabad en_US
dc.title ELECTROPHORETIC PATTERN OF POLYPEPTIDES OF INFECTIOUS BURSAL DISEASE VACCINE VIRUS en_US
dc.type Article en_US


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