Abstract:
Monolayer indirect immunoperoxidase (liP) assays were developed for the improvement of routine viral isolation and titration. The instant identification of virus with increased sensitivity ( 10-100 fold) was the characteristic of liP. Polyclonal chicken antiserum reacted with all the avian adenoviruses (FA V) strains studied. Viral isolation and titration was made possible without the need of blind passages. However, the isolates were also characterized or typed by studying their reaction pattern with a panel of monoclonal antibodies (MAbs) produced to FA V strain 398A.
