INDIRECT HEMAGGLUTINATION TEST FOR THE DETECTION OF ANTIBODIES AGAINST BOOPHILUS MICROPLUS

C.S. Hayat; Masood Akhtar; Iftikhar Hussain; Rehan Ahmad

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dc.contributor.author	C.S. Hayat
dc.contributor.author	Masood Akhtar
dc.contributor.author	Iftikhar Hussain
dc.contributor.author	Rehan Ahmad
dc.date.accessioned	2023-03-09T04:05:32Z
dc.date.available	2023-03-09T04:05:32Z
dc.date.issued	1999-07-11
dc.identifier.citation	Hayat, C. S., Akhtar, M., Hussain, I., & Ahmad, R. (1999). Indirect hemagglutination test for the detection of antibodies against Boophilus microplus. PAKISTAN VETERINARY JOURNAL, 19(3), 155-157.	en_US
dc.identifier.issn	0253-8313
dc.identifier.uri	http://142.54.178.187:9060/xmlui/handle/123456789/18069
dc.description.abstract	Indirect hemagglutination (IHA) test for the detection of antibodies against Boophilus (B.) microplus was developed. Midgut, B. microplus, antigen was used to sensitize the human group "0" erythrocytes without coupling agent. The optimum temperature for the sensitization of erythrocytes was found to be 37°C. The sensitizing dose of the antigen was calculated as 2.0 mL per 0.01 mL of the packed erythrocytes, diluted in 2 mL phosphate buffered saline, at 37°C for 60 minutes. The IHA results were best seen after 40 minutes of adding the sensitized erythrocytes in the serially diluted samples. IHA was found to be simple, rapid and inexpensive test for the detection of anti-tick antibodies.	en_US
dc.language.iso	en	en_US
dc.publisher	Faisalabad: Faculty of Veterinary Science University of Agriculture Faisalabad	en_US
dc.title	INDIRECT HEMAGGLUTINATION TEST FOR THE DETECTION OF ANTIBODIES AGAINST BOOPHILUS MICROPLUS	en_US
dc.type	Article	en_US