Abstract:
Alkaline phosphatase (AlkP 3.1.3.1) activity of sandy loam soil using pnitrophenyl phosphate, as substrate was determined. Maximum phosphatase activity was observed at 40°C and was significantly and positively correlated with temperature of incubation. The highest activity with buffer solution of pH 11 was also significantly and positively correlated. The activity was a linear function of incubation time up to 6 hrs and
then it decreased. Maximum activity was observed at field capacity and was a quadratic function of moisture contents.
