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GENETIC DIVERSITY OF APICAL MEMBRANE ANTIGEN (AMA-1) IN ISOLATES OF PLASMODIUM VIVAX FROM BALOCHISTAN.

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dc.contributor.author A. IJAZ
dc.contributor.author G. Panezai
dc.contributor.author S. Afridi
dc.contributor.author N. Ahmed
dc.contributor.author N. Rafique
dc.contributor.author B. Zohra
dc.contributor.author S. Saddozai
dc.date.accessioned 2023-03-14T05:52:39Z
dc.date.available 2023-03-14T05:52:39Z
dc.date.issued 2017-12-04
dc.identifier.citation Ijaz, A. (2017). GENETIC DIVERSITY OF APICAL MEMBRANE ANTIGEN (AMA-1) IN ISOLATES OF PLASMODIUM VIVAX FROM BALOCHISTAN. Pakistan Journal of Science, 69(4). en_US
dc.identifier.issn 0030-9877
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19023
dc.description.abstract Malaria is endemic in Pakistan but little research data is available on vaccine candidate antigens like Apical membrane antigen 1 (AMA-1), Circumsporozoite protein (CSP), Merozoite surface protein 1 (MSP-1) and Duffy binding protein. We found scanty information on genetic diversity of these antigen genes in Plasmodium species of Balochistan. The AMA-1 is one of the leading vaccine candidate antigen and required for the nvasion of red blood cells by the malaria parasites. To the best of our knowledge, this is the first study ocusing the genetic diversity of Plasmodium vivax AMA-1 (Pvama-1) gene among Pakistani isolates. The AMA-1 gene was amplified at domain-1 in 62 isolates out of 100 symptomatic clinical cases collected from Quetta, Balochistan. The amplified PCR products were further verified by sequence analysis of selected isolates. The results indicated that Plasmodium vivax showed considerable degree of polymorphism at AMA-1 gene domain-1. The comparison of PvAMA1 nucleotide sequences and resulting amino acid sequences with equences from other countries also showed a variation. This study will serve as base line data in this region for further molecular study of this gene from other parts of country as well. en_US
dc.language.iso en en_US
dc.publisher Lahore: Pakistan Association for the Advancement of Science en_US
dc.subject Malaria en_US
dc.subject Vaccine candidate gene en_US
dc.subject Polymorphism en_US
dc.subject AMAl en_US
dc.subject Plasmodium vivax en_US
dc.title GENETIC DIVERSITY OF APICAL MEMBRANE ANTIGEN (AMA-1) IN ISOLATES OF PLASMODIUM VIVAX FROM BALOCHISTAN. en_US
dc.type Article en_US


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