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EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR

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dc.contributor.author A. Hifza
dc.contributor.author M. I. Tariq
dc.contributor.author N. Sultana
dc.contributor.author T. Akhtar
dc.contributor.author S. Nawaz
dc.date.accessioned 2023-03-14T06:14:43Z
dc.date.available 2023-03-14T06:14:43Z
dc.date.issued 2017-06-20
dc.identifier.citation Hifza, A. (2017). EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR. Pakistan Journal of Science, 69(2). en_US
dc.identifier.issn 0030-9877
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19103
dc.description.abstract : Prunus Arminica.L seeds of Hunza cultivar were defatted with chloroform, methanol mixture (2:1v/v) by ultrasonic assisted extraction technique. The lipase and phospholipase extracted, incubated at 400C and pH 6 in citrate buffer for 1 hour. The lipolytic activities of enzymes were measured at different temperatures i,e 30-700C , pH i,e 3-9 and solvents i,e n-heptane , Di-isopropyl ether and Cyclo hexane. 10 % emulsion of lecithin and olive oil used as the substrate for phospholipase and lipase substrates respectively. The enzymes showed stability at lower temperatures i,e 35-500C and slightly acidic to neutral i.e pH 5 to pH 7. Concentration of liberated fatty acids was the indicator of the enzymes activity which were measured by taking absorbance at 440nm. Stearic acid with different concentrations in hexane and methanol (1:1v/v) were used for the standard curve. Cu-TEA reagent with small quantity of CaCl2 was used for maximum extraction of Cu-FFA and for stable value of blanks in the present study. The enzymes showed maximum activities at 450 C temperature and 6 pH en_US
dc.language.iso en en_US
dc.publisher Pakistan Association for the Advancement of Science,Pakistan en_US
dc.subject Ultrasonic extraction en_US
dc.subject Prunus Arminica en_US
dc.subject Olive oil en_US
dc.subject Lecithin, Lipase en_US
dc.subject Stearic acid Phospholipase en_US
dc.title EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR en_US
dc.type Article en_US


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