dc.contributor.author |
A. Hifza |
|
dc.contributor.author |
M. I. Tariq |
|
dc.contributor.author |
N. Sultana |
|
dc.contributor.author |
T. Akhtar |
|
dc.contributor.author |
S. Nawaz |
|
dc.date.accessioned |
2023-03-14T06:14:43Z |
|
dc.date.available |
2023-03-14T06:14:43Z |
|
dc.date.issued |
2017-06-20 |
|
dc.identifier.citation |
Hifza, A. (2017). EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR. Pakistan Journal of Science, 69(2). |
en_US |
dc.identifier.issn |
0030-9877 |
|
dc.identifier.uri |
http://142.54.178.187:9060/xmlui/handle/123456789/19103 |
|
dc.description.abstract |
: Prunus Arminica.L seeds of Hunza cultivar were defatted with chloroform, methanol
mixture (2:1v/v) by ultrasonic assisted extraction technique. The lipase and phospholipase extracted,
incubated at 400C and pH 6 in citrate buffer for 1 hour. The lipolytic activities of enzymes were
measured at different temperatures i,e 30-700C , pH i,e 3-9 and solvents i,e n-heptane , Di-isopropyl
ether and Cyclo hexane. 10 % emulsion of lecithin and olive oil used as the substrate for phospholipase
and lipase substrates respectively. The enzymes showed stability at lower temperatures i,e 35-500C and
slightly acidic to neutral i.e pH 5 to pH 7. Concentration of liberated fatty acids was the indicator of
the enzymes activity which were measured by taking absorbance at 440nm. Stearic acid with different
concentrations in hexane and methanol (1:1v/v) were used for the standard curve. Cu-TEA reagent
with small quantity of CaCl2 was used for maximum extraction of Cu-FFA and for stable value of
blanks in the present study. The enzymes showed maximum activities at 450 C temperature and 6 pH |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Pakistan Association for the Advancement of Science,Pakistan |
en_US |
dc.subject |
Ultrasonic extraction |
en_US |
dc.subject |
Prunus Arminica |
en_US |
dc.subject |
Olive oil |
en_US |
dc.subject |
Lecithin, Lipase |
en_US |
dc.subject |
Stearic acid Phospholipase |
en_US |
dc.title |
EFFECTS OF DIFFERENT PARAMETERS ON THE LIPOLYTIC ACTIVITIES OF LIPASE AND PHOSPHOLIPASE EXTRACTED FROM PRUNUS ARMINICA MEAL OF HUNZA CULTIVAR |
en_US |
dc.type |
Article |
en_US |