Abstract:
This study was designed to evaluate the supplementation of various concentrations
of selenium and vitamin E and combinations in lactose egg-yolk-glycerol based extender on
cryosurvival of buffalo bull semen. The semen of four healthy donor bulls and ejaculates (n= 80) were
processed and pooled. The collected semen was extended with the experimental extenders and then
frozen. The frozen straws of semen were thawed and analyzed for motility, viability, acrosomal
integrity and functional integrity of sperm. The study indicated that combined use of selenium and
vitamin E @ 2μg/ml + 0.75 mg/ml supplemented in extender had significantly (P<0.05) increased
sperm motility, viability, acrosomal integrity and functional membrane integrity. Selenium (2μg/ml) in
freezing extender also improved semen cryosurvival. The provision of selenium (3μg/ml) alone and in
combination with Vitamin E (1 mg/ml) did not provide any beneficial impact on sperm cryosurvival. It
was concluded that addition of selenium and vitamin E alone in fructose lactose egg yolk glycerol
extender increased the cryosurvival of buffalo sperms
