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ROLE OF SELENIUM AND VITAMIN E IN LACTOSE BASED EXTENDER ON SEMEN CRYOPRESERVATION OF BUFFALO BULL (BUBALUS BUBALIS)

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dc.contributor.author M. Ali
dc.contributor.author M. Ahmad
dc.contributor.author S.T. Gul
dc.contributor.author M. Zubair
dc.contributor.author I. Ahmad
dc.contributor.author S. Ali
dc.date.accessioned 2023-03-14T06:16:33Z
dc.date.available 2023-03-14T06:16:33Z
dc.date.issued 2017-06-20
dc.identifier.citation Ali, M. (2017). Role of selenium and vitamin E in lactose based extender on semen cryopreservation of buffalo bull (bubalus bubalis). Pakistan Journal of Science, 69(2). en_US
dc.identifier.issn 0030-9877
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19110
dc.description.abstract This study was designed to evaluate the supplementation of various concentrations of selenium and vitamin E and combinations in lactose egg-yolk-glycerol based extender on cryosurvival of buffalo bull semen. The semen of four healthy donor bulls and ejaculates (n= 80) were processed and pooled. The collected semen was extended with the experimental extenders and then frozen. The frozen straws of semen were thawed and analyzed for motility, viability, acrosomal integrity and functional integrity of sperm. The study indicated that combined use of selenium and vitamin E @ 2μg/ml + 0.75 mg/ml supplemented in extender had significantly (P<0.05) increased sperm motility, viability, acrosomal integrity and functional membrane integrity. Selenium (2μg/ml) in freezing extender also improved semen cryosurvival. The provision of selenium (3μg/ml) alone and in combination with Vitamin E (1 mg/ml) did not provide any beneficial impact on sperm cryosurvival. It was concluded that addition of selenium and vitamin E alone in fructose lactose egg yolk glycerol extender increased the cryosurvival of buffalo sperms en_US
dc.language.iso en en_US
dc.publisher Pakistan Association for the Advancement of Science,Pakistan en_US
dc.subject Selenium en_US
dc.subject Vitamin E en_US
dc.subject Buffalo bull semen en_US
dc.subject Extender en_US
dc.title ROLE OF SELENIUM AND VITAMIN E IN LACTOSE BASED EXTENDER ON SEMEN CRYOPRESERVATION OF BUFFALO BULL (BUBALUS BUBALIS) en_US
dc.type Article en_US


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