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Development and Validation of Reversed-Phase High Performance Liquid Chromatographic Method for Analysis of Cephradine in Human Plasma Samples

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dc.contributor.author MAHMOOD AHMAD
dc.contributor.author MUHAMMAD USMAN
dc.contributor.author ASADULLAH MADNI
dc.contributor.author NAVEED AKHTAR
dc.contributor.author NAYAB KHALID
dc.contributor.author WAHEED ASGHAR
dc.contributor.author SATTAR BAKHSH
dc.date.accessioned 2023-09-26T03:36:13Z
dc.date.available 2023-09-26T03:36:13Z
dc.date.issued 2010-02-20
dc.identifier.citation Ahmad, M., Usman, M., Madni, A., Akhtar, N., Khalid, N., & Asghar, W. (2010). Development and validation of reversed-phase high performance liquid chromatographic method for analysis of cephradine in human plasma samples. Journal of the Chemical Society of Pakistan, 32(1), 58-63. en_US
dc.identifier.issn 0253-5106
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19697
dc.description.abstract An HPLC method with high precision, accuracy and selectivity was developed and validated for the assessment of cephradine in human plasma samples. The extraction procedure was simple and accurate with single step followed by direct injection of sample into HPLC system. The extracted cephradine in spiked human plasma was separated and quantitated using reversed phase C18 column and UV detection wavelength of 254 nm. The optimized mobile phase of new composition of 0.05 M potassium dihydrogen phosphate (pH 3.4)-acetonitrile (88:12) was pumped at an optimum flow rate of 1 mL.min -1 . The method resulted linearity in the concentration range 0.1520 µg.mL-1The limit of detection (LOD) and limit of quantification (LOQ) were 0.05 and 0.150 µg.mL-1 , respectively. The accuracy of method was 98.68 %. This method can be applied for bioequivalence studies and therapeutic drug monitoring as well as for the routine analysis of cephradine. en_US
dc.language.iso en en_US
dc.publisher HEJ Research Institute of Chemistry, University of Karachi, Karachi. en_US
dc.title Development and Validation of Reversed-Phase High Performance Liquid Chromatographic Method for Analysis of Cephradine in Human Plasma Samples en_US
dc.type Article en_US


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