STRUCTURE ELUCIDATION OF CHROMOGEN RESULTING FROM JAFFE'S REACTION

dc.contributor.author	ISHTIAQ HUSSAIN
dc.contributor.author	MUHAMMAD ILYAS TARIQ
dc.contributor.author	HAMID LATIF SIDDIQUI
dc.date.accessioned	2023-09-26T07:50:27Z
dc.date.available	2023-09-26T07:50:27Z
dc.date.issued	2009-12-16
dc.identifier.citation	Hussain, I., Tariq, M. I., & Siddiqui, H. L. (2009). Structure elucidation of chromogen resulting from Jaffe's reaction. Journal of the Chemical Society of Pakistan, 31.	en_US
dc.identifier.issn	0253-5106
dc.identifier.uri	http://142.54.178.187:9060/xmlui/handle/123456789/19704
dc.description.abstract	Jaffe’s reaction is a reaction of creatinine with alkaline picric acid resulting in red chromogen. This color is being used widely for the estimation of creatinine in biological fluids without knowing the exact structure of the chromogen. The red species is isolated and its UV/Visible, 1H- and 13C- NMR studies are carried out. It is found that the red species is not a pure compound but a mixture of stereoisomers. Moreover, the red species in water reflects the same UV/Visible spectra as given by the reaction of creatinine with alkaline picrate in aqueous media. The red species is a 2:3 molar Meisenheimer σ-complex of creatinine-picric acid (6)	en_US
dc.description.sponsorship	The chemical society of Pakistan is an approved society from the PSF.	en_US
dc.language.iso	en	en_US
dc.publisher	The Pakistan Society Of Biochemists	en_US
dc.title	STRUCTURE ELUCIDATION OF CHROMOGEN RESULTING FROM JAFFE'S REACTION	en_US
dc.type	Article	en_US