Abstract:
Capillary gas chromatographic separation of putrescine (Pu), cadaverine (Cd), dopamine (DA) and octopamine (OA) as derivatives of trifluoroacetylacetone (FAA) has been carried out using a column HP-5 (30 m × 0.32 mm id), temperature 100 ºC for 1 min, followed by heating rate 10 ºC/min up to 250 ºC using FID detection. Nitrogen flow rate was 4 mL/min and split ratio was 10:1 v/v. The linear calibration graphs were ranging between 2.5-25 μg/mL. The method was applied for the determination dopamine in pharmaceutical preparations with relative standard deviation (RSD) within 1.5-2.5% and recovery of 98% with RSD 1%.
