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A Cystein Protease from Asteraceae (Centaurea Aegialophila)

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dc.contributor.author NAZAN DEMİR
dc.contributor.author HAYRUNNISA NADAROĞLU
dc.contributor.author MUHARREM TOPAL
dc.contributor.author YAŞAR DEMİR
dc.date.accessioned 2023-10-09T04:30:21Z
dc.date.available 2023-10-09T04:30:21Z
dc.date.issued 2011-10-19
dc.identifier.citation Demir, N., NADAROĞLU, H., Topal, M., & Demir, Y. (2011). A cystein protease from Asteraceae (Centaurea aegialophila). Journal of The Chemical Society of Pakistan, 33(5). en_US
dc.identifier.issn 0253-5106
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19816
dc.description.abstract Proteases are enzymes that perform very important functions in organisms and are used for a variety of objectives in vitro. In recent years, proteases have been used for clinical, pharmaceutical (alimentary digestion, anti-inflammatory, etc.) and industrial applications (cheese production, meat tenderizing, leather tanning). In this research, a protease has been purified from capsules of flowers of asteraceae (Centaurea aegialophila) and characterized. Asteraceae plants have been used for food and medicine since ancient times. The plant grows abundantly in certain regions of Turkey. Ammonium sulphate fractionation and a CM Sephadex column were used for purification of the enzyme. The purified enzyme has an optimum pH=5.0 and its optimum temperature was 70 °C. The Vmax and KM values were determined by Lineweaver-Burk graphics using different substrate. The purification degree and the molecular mass of the enzyme (25 kDa) were determined by SDSPAGE and gel filtration chromatography. It was investigated whether the purified and characterized protease could cause milk to congeal or digest chicken and cow meat. The results show that protease can be used for industrial production. en_US
dc.description.sponsorship The chemical society of Pakistan is an approved society from the PSF. en_US
dc.language.iso en en_US
dc.publisher Karachi: The Chemical Society Of Pakistan en_US
dc.title A Cystein Protease from Asteraceae (Centaurea Aegialophila) en_US
dc.type Article en_US


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