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Ethyl Chloroformate as Derivatizing Reagent for Gas Chromatographic Determination of α-Keto acids and Amino acids from Pharmaceutical Preparation

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dc.contributor.author KULSOOM UBEDULLAH ABBASI
dc.contributor.author MUHAMMAD YAR KHUHAWAR
dc.contributor.author MUHAMMAD IQBAL BHAMGER
dc.contributor.author KHALIDA PARVEEN MAHAR
dc.contributor.author ABDUL HAMEED CHANNAR
dc.date.accessioned 2023-10-13T07:29:18Z
dc.date.available 2023-10-13T07:29:18Z
dc.date.issued 2011-10-30
dc.identifier.citation MI, K. A., & Khuhawar, M. Y. (2009). Ethyl chloroformate as derivatizing reagent for gas chromatographic determination of norephedrine, ephedrine and pseudoephedrine in pharmaceutical preparation. Asian Journal of Chemistry, 21(2), 1145. en_US
dc.identifier.issn 0253-5106
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19838
dc.description.abstract α-keto acids pyruvic acid (PYR), 2-ketobutyric acid (KBA), 3-methyl-2-oxobutyric acid (MKBA), 3- methyl-2-oxovalaric acid M3KVA), 4- methyl-2-oxovalaric acid (M4KVA), 2- ketohexanoic acid (KHA) and phenylpyruvic acid (PPYR) together with amino acids as derivatives of ethyl chloroformate (ECF) eluted and separated from GC column HP-5(30m x 0.32 mm id). The detection was by flame ionization ( (FID). Linear calibration curves were obtained within 18-127.8 µg/mL and detection limits were within 6-8.6 µg/mL of each α-keto acid. The separation was repeatable with inter and intraday variation with relative standard deviation (RSD) within 0.2-3.5%. A pharmaceutical preparation Ketosteril was analyzed and amounts of MKBA, M3KVA, M4KVA, and PPYR correlated with labeled values and their amount extracted from pharmaceutical preparation by standard addition were calculated to 93.92 -96.1 % with RSD 0.3-0.7%. The amino acids present in Ketosteril tablet separated completely and were determined simultaneously together with α-keto acids with recovery of 95.8 to 98.65% with RSD 0.2-0.9%. en_US
dc.description.sponsorship The chemical society of Pakistan is an approved society from the PSF. en_US
dc.language.iso en en_US
dc.publisher Karachi: The Chemical Society Of Pakistan en_US
dc.title Ethyl Chloroformate as Derivatizing Reagent for Gas Chromatographic Determination of α-Keto acids and Amino acids from Pharmaceutical Preparation en_US
dc.type Article en_US


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