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New High Performance Liquid Chromatographic Method for Simultaneous Determination of Diclofenac and Meloxicam in Oral Formulation of Liposomes and Human Plasma

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dc.contributor.author ASADULLAH MADNI
dc.contributor.author MAHMOOD AHMAD
dc.contributor.author MUHAMMAD USMAN
dc.contributor.author MALIK MUHAMMAD ZUBAIR
dc.contributor.author MUHAMMAD QAMAR-UZ-ZAMAN
dc.contributor.author HAJI MUHAMMAD SHOAIB
dc.contributor.author ABUBAKAR MUNIR
dc.contributor.author SHUJAT ALI KHAN
dc.contributor.author MUHAMMAD NAEEM AMIR
dc.contributor.author MUHAMMAD SALEEM QURESHI
dc.date.accessioned 2023-11-06T04:21:56Z
dc.date.available 2023-11-06T04:21:56Z
dc.date.issued 2010-10-20
dc.identifier.citation Madni, A., Ahmad, M., Usman, M., Zubair, M. M., Shoiab, H. M., Khan, S. A., & Qureshi, M. S. (2010). New high performance liquid chromatographic method for simultaneous determination of diclofenac and meloxicam in oral formulation of liposomes and human plasma. Journal of the chemical society of Pakistan, 32(5), 654-661. en_US
dc.identifier.issn 0253-5106
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19910
dc.description.abstract A new, easy and consistent reversed-phase high-performance liquid chromatographic method with diode array detection has been developed and validated for the simultaneous determination of diclofenac potassium (DP) and meloxicam (MLX) in liposome, marketed brands and human plasma. Microextraction method based on liquid-liquid extraction principal was used to extract drug from plasma matrix. The optimized mobile phase was used in the molar ratio of 20:20:60 (v/v/v) mixture of acetonitrile, methanol and 20 x 10⁻³ M potassium dihydrogen phosphate buffer (pH 3.7), pumped at an optimized flow rate of 1.0 mL.min⁻¹. The linearity was performed in the concentration range of 15 ng.mL⁻¹ to 10µg.mL⁻¹ with r² of 0.9989 ± 0.13 and 0.9979 ± 0.11 (n = 6) for DP and MLX, respectively. The assay was repeatable at concentration levels of 10 ng.mL⁻¹, 1 µg.mL⁻¹ and 10 µg.mL⁻¹ with coefficient of variation of 0.168 - 0.603% for 10 ng.mL⁻¹ (DP), 15 ng.mL⁻¹ (MLX) and 1 µg.mL⁻¹ &10 µg.mL⁻¹ for DP and MLX. The LOD values were 3 and 5 ng.mL⁻¹, while values of LOQ were 10 and 15 ng.mL⁻¹, for DP and MLX. The present method is applicable in routine quality control operations, advanced drug delivery formulations (Liposomes) and in human Plasma samples for pharmacokinetics and bioequivalence studies. en_US
dc.description.sponsorship The chemical society of Pakistan is an approved society from the PSF. en_US
dc.language.iso en en_US
dc.publisher HEJ Research Institute of Chemistry, University of Karachi, Karachi. en_US
dc.title New High Performance Liquid Chromatographic Method for Simultaneous Determination of Diclofenac and Meloxicam in Oral Formulation of Liposomes and Human Plasma en_US
dc.type Article en_US


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