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Development of High Performance Liquid Chromatographic Method with Pre-Column Derivatization for Determination of Enantiomeric Purity of Hydantoin-5-acetic Acid

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dc.contributor.author DAO CAI WANG
dc.contributor.author YU CAO
dc.contributor.author ZHI JIAN HE
dc.contributor.author SHUN YAO
dc.contributor.author HANG SONG
dc.date.accessioned 2023-11-21T04:21:27Z
dc.date.available 2023-11-21T04:21:27Z
dc.date.issued 2013-08-02
dc.identifier.citation Wang, D. C., Yao, S., & Song, H. (2013). Development of high performance liquid chromatographic method with pre-column derivatization for determination of enantiomeric purity of hydantoin-5-acetic acid. Journal of the Chemical Society of Pakistan, 35. en_US
dc.identifier.issn 0253-5106
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19958
dc.description.abstract The objective of the present work was to develop and validate a rapid analytical method for determination of enantiomeric purity of hydantoin-5-acetic acid. The method was based on pre-column derivatization of hydantoin-5-acetic acid with phenylamine and complete separation of enantiomers has been achieved on a CHI-DMB analytical column (250×4.6 mm) using n-hexane: isopropanol (80:20 v/v) as mobile phase at a flow rate of 1.0 ml min-1 under UV and optical rotation (OR) detection. Detection wavelength was set at 254 nm. The effects of isopropanol, ethanol, column temperature and flow rate of the mobile phase on enantioselectivity and resolution of enantiomers were evaluated. The method was validated with respect to precision, accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ), robustness and stability. The present method is expected to be accurate, stable, rapid and sensitive and can be used for the determination of the enantiomeric purity of hydantoin-5-acetic acid in bulk samples. en_US
dc.description.sponsorship The chemical society of Pakistan is an approved society from the PSF. en_US
dc.language.iso en en_US
dc.publisher HEJ Research Institute of Chemistry, University of Karachi, Karachi. en_US
dc.subject HPLC en_US
dc.subject enantiomeric purity en_US
dc.subject enantioseparation en_US
dc.subject hydantoin-5-acetic acid en_US
dc.subject pre-column derivatization en_US
dc.subject method validation en_US
dc.title Development of High Performance Liquid Chromatographic Method with Pre-Column Derivatization for Determination of Enantiomeric Purity of Hydantoin-5-acetic Acid en_US
dc.type Article en_US


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