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Determination of Estrogens in Milk by HPLC using Salting-Out Extraction

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dc.contributor.author DEQING ZOU
dc.contributor.author YAN ZOU
dc.contributor.author ZHIGANG TAI
dc.contributor.author QI WANG
dc.contributor.author YALING YANG
dc.date.accessioned 2023-11-23T05:36:41Z
dc.date.available 2023-11-23T05:36:41Z
dc.date.issued 2013-08-04
dc.identifier.citation Zou, D., Zou, Y., & Tai, Z. (2013). Determination of estrogens in milk by hplc using salting-out extraction. Journal of the Chemical Society of Pakistan, 35(4), 1160-1167. en_US
dc.identifier.issn 0253-5106
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/19965
dc.description.abstract A simple salting-out assisted liquid–liquid extraction (SALLE) method with acetonitrile combined with high-performance liquid-chromatography was developed and applied for the determination four kinds of estrogens (estradiol (βE2), diethylstilbestrol (DES), estrone (E1) and progesterone (PES)) in milk. The influences of effective parameters such as salt (type and amount), water-miscible organic solvent, phase volume, equilibration time, centrifugation time and pH of sample solution were all studied and optimized. Under optimized experimental conditions, calibration plots were found to be linear in the range of 0.01–5 mg/l for E1, DES and βE2, and 0.02-8 mg/l for PES, with coefficient of determinations more than 0.996, preconcentration factors of 85-89 for those estrogens in milk. The relative recoveries ranged over 95.1-102.5%, the relative standard deviations (RSD) were 0.3-4.4% (n =5). And the limits of quantification achieved with this method were 0.4×10⁻³ mg/l for βE2, 0.7×10⁻³ mg/l for E1, 0.65×10⁻³mg/l for DES and 2.4×10⁻³ mg/l for PES, respectively. en_US
dc.description.sponsorship The chemical society of Pakistan is an approved society from the PSF. en_US
dc.language.iso en en_US
dc.publisher HEJ Research Institute of Chemistry, University of Karachi, Karachi. en_US
dc.subject Estrogens en_US
dc.subject Salting-out extraction en_US
dc.subject High Performance Liquid Chromatography en_US
dc.subject Milk, Preconcentration, Acetonitrile. en_US
dc.title Determination of Estrogens in Milk by HPLC using Salting-Out Extraction en_US
dc.type Article en_US


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