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Ultrasonically Assisted Extraction and HPLC Determination of Chondroitin Sulfate from Fish Heads

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dc.contributor.author CHUN LIN KE
dc.contributor.author XIAO XIONG ZENG
dc.date.accessioned 2023-12-05T04:54:55Z
dc.date.available 2023-12-05T04:54:55Z
dc.date.issued 2012-06-20
dc.identifier.citation Ke, C. L., & Zeng, X. X. (2012). Ultrasonically Assisted Extraction and HPLC Determination of Chondroitin Sulfate from Fish Heads. Journal of the Chemical Society of Pakistan, 34(6), 557. en_US
dc.identifier.issn 0253-5106
dc.identifier.uri http://142.54.178.187:9060/xmlui/handle/123456789/20002
dc.description.abstract In the present study, we investigated the ultrasonically assisted extraction(UAE) and high-performance liquid chromatography (HPLC) determination of chondroitin sulfate(CS) from fish heads. Firstly, the optimized parameters for CS extraction were obtained by using Box– Behnken design(BBD) and response surface methodology(RSM) based on the single-factor experiments. As results, the optimum conditions were ultrasonic time 45min, ultrasonic power 280W and solvent/material ratio 6ml/g. Under these conditions, the experimental yield of CS was 4.623%. Secondly, the CS was purified by DEAE-Cellulose 52 chromatography and Sephadex G100 chromatography to afford purified CS. Finally, we developed a simple method for the determination of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) in purified CS by HPLC with a diode array detector (DAD). The results showed that there were obvious saccharides peaks in CS. en_US
dc.description.sponsorship The chemical society of Pakistan is an approved society from the PSF. en_US
dc.language.iso en en_US
dc.publisher HEJ Research Institute of Chemistry, University of Karachi, Karachi. en_US
dc.title Ultrasonically Assisted Extraction and HPLC Determination of Chondroitin Sulfate from Fish Heads en_US
dc.type Article en_US


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