Abstract:
Many germplasm of Pisum sativum have been screen for estimation of genetic diversity, cluster
plating and gene linkages. Little attention has been given to the development of homozygous
lines from single seed descent method in pea. In the present study 24 pea lines were evaluated,
out of 24 lines, 20 pre-developed novel lines of Pisum sativum were achieved through single
seed descent method from the cross of Falloner and 11760-3ER. In parental combination, Falloner
(female) was dwarf, bushy tendrils, leafless, no anthocyanin pigmentation, creamy flower, green
pod, creamy seed color, smooth seed shape, disease resistant and low yielding. Whereas, 11760-
3ER (male) was tall, normal tendrils, leaflet present, anthocyanin pigmentation present, purple
flower, purple pod, brown seed color, rough seed shape, disease susceptible and high yielding.
The control lines for comparative performance was Climex (PL-22) and local cultivar (PL-21).
All the selected pea lines were evaluated through morphological and molecular markers in order
to select high yielding and powdery mildew resistant novel lines. These lines were further
screened for estimation of genetic diversity and genetic linkages. In morphological traits analysis
9 qualitative and 22 quantitative traits were studied. Based on quantitative traits analysis, two
novel high yielding and disease resistant pea lines (PL-4 and PL-5) were selected for seed
certification. The grain yield of PL-4 and PL-5 is significantly (p< 0.05) high as compared to PL-
21 and PL-22. Regarding traits association, "seed coat with anthocyanin pigmentation" showed
genetic linkage with "pigmentation on flower and leaf axil" and vice versa. Interestingly, the
cluster analysis grouped "tall/low yielding", "dwarf/low yielding", "tall/high yielding",
"dwarf/high yielding" pea lines independently. For molecular characterization, 20 RAPD and 85
SSR markers were screened to estimate genetic diversity and genetic linkage. The RAPD
markers amplified 69 bands out of which 95.6% bands were polymorphic. The similarity indexes
were ranging from 0.43 to 0.88%. Furthermore, out of 85 SSR markers, 12 showed genetic
polymorphism and produced an average of 2 alleles per SSR locus. The polymorphism
information content (PIC) values of SSR markers ranged from 0.08 to 0.47, with an average of
0.33. Nei’s genetic distance (GD) between accessions ranged from 0.10 to 0.92. Among the total
used SSR primers, only P628 SSR marker showed significant linkage with grain yield traits at
90.91% probability. The linkage of the marker was reconfirmed through Chi-square test and
showed r = 0.918 (P ≤0.00) genetic linkage. This markers can be directly utilized in MAS
breeding for the selection of high yielding edible pea lines.