Abstract:
The presented research work in this dissertation comprises of synthesis,
characterization, antimicrobial, hemolytic and enzyme inhibition studies of some new
acetamides/sulfides bearing substituted-1,3,4-Oxadiazole and morpholine moieties.
The
acetamide,
1,3,4-oxadiazole
and
morpholine
moieties
are
important
functionalities because of their broad range of known pharmacological activities.
Synthetic approaches (scheme-1-11) were utilized to synthesize poly-functional
compounds. In first scheme, twenty three 5-substituted-1,3,4-Oxadiazol-2-thiols were
synthesized by converting multifarious organic acids consecutively into the
corresponding esters and hydrazides. Further the intermolecular cyclization of various
carbohydrazides with carbon disulfide and potassium hydroxide yielded subsequent 5-
substituted-1,3,4-Oxadiazol-2-thiols.
Moreover,
the
reaction
of
different
5-
substituted-1,3,4-Oxadiazol-2-thiols (scheme-2 & 3) with electrophiles, 2-bromo-N-
[4-(4-morpholinyl)phenyl]acetamide
and
2-bromo-N-[2-(4-morpholinyl)phenyl]
acetamide yielded thirteen, N-[4-(4-morpholinyl)phenyl]-2-[(5-aryl/aralkyl-1,3,4-
Oxadiazol-2-yl)thio]acetamides and fourteen N-[2-(4-morpholinyl)phenyl]-2-[(5-
aryl/aralkyl-1,3,4-oxadiazol-2-yl)thio]acetamides respectively in the presence of N,N-
dimethylformamide and sodium hydride.
Sixteen novel benzyl sulfides (scheme-4) bearing 1,3,4-oxadiazole moiety and sulfo-
morpholine
functionality
were
synthesized
(bromomethyl)phenyl)sulfonyl)morpholine
with
by
the
different
reaction
of
4-(4-
5-substituted-1,3,4-
Oxadiazole-2-thiols. Nucleophilic substitution reaction of free thiol group in 1,3,4-
Oxadiazoles with 4-(2-chloroethyl)morpholine hydrochloride yielded thirteen, 4-[2-
[[5-aryl/aralkyl-1,3,4-Oxadiazol-2-yl]thio]ethyl]morpholine derivatives (scheme-5) in
the presence of acetone and potassium carbonate .
A series of nineteen electrophiles (scheme-5), N-substituted-2-bromoacetamides was
developed by the reaction of different substituted/unsubstituted aryl/aralkyl/alkyl
amines with 2-bromoacetyl bromide by using DCM or basic aqueous medium as
solvent.
Seventy one (71) acetamides were synthesized by the reaction of 5-(2-chloro/3-
chloro/4-chloro/4-nitrophenyl/phenyl)-1,3,4-Oxadiazol-2-thiols
with
different
electrophiles by simple stirring in the presence of DMF solvent and NaH base at the
room temperature.
The structures of all the synthesized compounds were characterized by using IR, 1H-
NMR and mass spectral data. In addition, 13C-NMR technique was also used in some
cases to support the structural analysis. A rational mass fragmentation pattern of some
of the compounds is also proposed. Some of the 1H-NMR,
13
C-NMR and EIMS
spectra of synthesized compounds are also presented for the obvious perceptive of
signals. The synthesized compounds were evaluated for antibacterial, antifungal,
hemolytic and enzyme inhibition activities. Some of compounds were found to be
active and showed interesting results in different studies declared above. The
biological activity data in comparison of each scheme with the reference standard
drugs is presented in biological activity section.